JCRB0071 BALL-1

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0071 Cell Name   BALL-1
Profile   Acute lymphoblastoid leukemia (ALL) cell line from human. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   acute lymphoblastoid leukemia (ALL) Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   
Classify   tumor Established by   Hiraki,S.
Registered by   Akiyama,M. Regulation for Distribution   free
Comment    Year   1985
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   Dilution, twice a week.
Cell Number on Passage   split ratio=1/3 Race   
CO2 Conc.   5 % Tissue Sampling   peripheral blood
Virus Summary   tested Virus Tested   CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-) [-/negative, +/positive, nt/not tested]
Tissue Type   ALL
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1075
Reference
Pubmed id:2698048 Expression of cytokine genes in hematological malignancies.
Okamura S,Hayashi S,Kawasaki C,Kondo S,Asano Y,Omori F,Otsuka T,Shibuya T,Harada M,Niho Y
Nihon Ketsueki Gakkai Zasshi. 1989 Dec;52(8):1423-32
Pubmed id:197412 Human B cell, T cell and null cell leukaemic cell lines derived from acute lymphoblastic leukaemias.
Miyoshi I,Hiraki S,Tsubota T,Kubonishi I,Matsuda Y,Nakayama T,Kishimoto H,Kimura I,Masuji H
Nature. 1977 Jun 30;267(5614):843-4

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LOT Information

Cell No.   JCRB0071 Cell Name   
LOT No.   09212012 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   3.4 x 10^5 cells/mL Methods for Passages   Simple dilution. (suspension culture)
Doubling Time   approx. 28 hrs Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability (%)   82.9 Antibiotics Used   free
Passage Number   Unknown (10 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Additional information   
Cell No.   JCRB0071 Cell Name   BALL-1
LOT No.   053193 Lot Specification   distribution
Medium   RPMI-1640 with 10% heat inactivated fetal bovine serum (Hyclone 115975) Temperature   37 C
Cell Density at Seeding   3.5x10^5 cells/ml Methods for Passages   Harvest cells by centrifuge and wash cells.
Doubling Time   NT Cell Number in Vial (cells/1ml)   5.75x10^4
Viability (%)   73.9 Antibiotics Used   free
Passage Number   P7 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Additional information   
Cell No.   JCRB0071 Cell Name   BALL-1
LOT No.   042190 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   dilution 1/3 Methods for Passages   Dilution, twice a week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.2x10^6
Viability (%)   NT Antibiotics Used   free
Passage Number   P5* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Additional information   
Cell No.   JCRB0071 Cell Name   BALL-1
LOT No.   100187 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   dilution 1/3 Methods for Passages   Dilution, twice a week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   10^6
Viability (%)   92% Antibiotics Used   free
Passage Number   P4* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Additional information   
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