JCRB0072 MiTen

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0072 Cell Name   MiTen
Profile   Human cell line from Lesch-Nyhan syndrome. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   skin
Case history   Lesh-Nyhan syndrome, genetic disease Metastasis   
Tissue Metastasized    Genetics   
Life Span   finite Crisis PDL   
Morphology   fibroblast-like Character   
Classify   normal Established by   Lesch,M. & Nyhan,W.
Registered by   Akiyama,M. Regulation for Distribution   
Comment    Year   
Medium   Eagle's minimal essentila medium with 10% fetal calf serum Methods for Passages   Treat cells with 0.25 % trypsin twice a week
Cell Number on Passage   split ratio=1/2-1/3 Race   
CO2 Conc.   5 % Tissue Sampling   skin
Virus Summary   tested Virus Tested   CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(nt) [-/negative, +/positive, nt/not tested]
Tissue Type   normal skin fibroblast
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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3024
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LOT Information

Cell No.   JCRB0072 Cell Name   MiTen
LOT No.   082391 Lot Specification   distribution
Medium   MEM + 10 % FCS Temperature   37 C
Cell Density at Seeding   dilution 1/2-1/3 Methods for Passages   0.25 % trypsin, twice a week
Doubling Time   NT Cell Number in Vial (cells/1ml)   8.2x10^5
Viability (%)   NT Antibiotics Used   free
Passage Number   P9 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Additional information   
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