JCRB0076 A549

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0076 Cell Name   A549
Profile   Human adeno carcinoma cell line derived from lung cancer. Other Name   A-549
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   58
Identity   available Tissue for Primary Cancer   lung (cancer)
Case history   lung cancer Metastasis   
Tissue Metastasized    Genetics   adenocarcinoma
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Y chr.invisible. Detected by Y specific DNA probe.
Classify   tumor Established by   Giard,D.
Registered by   Akiyama,M. Regulation for Distribution   free
Comment    Year   1985
Medium   Eagle's minimal essential medium with non essential amino acids and 10% fetal calf serum Methods for Passages   Cells are treated with 0.02 % EDTA and 0.25 % trypsin.
Cell Number on Passage   2x10^4 cells/sq.cm. Race   Caucasian
CO2 Conc.   5 % Tissue Sampling   lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0023
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 905949
Reference
Pubmed id:2783462 Inhibition of growth of human lung adenocarcinoma cell lines by anti-transforming growth factor-alpha monoclonal antibody.
Imanishi K,Yamaguchi K,Kuranami M,Kyo E,Hozumi T,Abe K
J Natl Cancer Inst. 1989 Feb 1;81(3):220-3
Pubmed id:175022 A continuous tumor-cell line from a human lung carcinoma with properties of type II alveolar epithelial cells.
Lieber M,Smith B,Szakal A,Nelson-Rees W,Todaro G
Int J Cancer. 1976 Jan 15;17(1):62-70


Research results by users. Click!
Pubmed id:28676715 Allantopyrone A interferes with multiple components of the TNF receptor 1 complex and blocks RIP1 modifications in the TNF-α-induced signaling pathway.
Quach HT,Tanigaki R,Yokoigawa J,Yamada Y,Niwa M,Hirano S,Shiono Y,Kimura KI,Kataoka T
J Antibiot (Tokyo). 2017 Aug;70(9):929-936
Pubmed id:29207489 Quinacrine Inhibits ICAM-1 Transcription by Blocking DNA Binding of the NF-κB Subunit p65 and Sensitizes Human Lung Adenocarcinoma A549 Cells to TNF-α and the Fas Ligand.
Harada M,Morimoto K,Kondo T,Hiramatsu R,Okina Y,Muko R,Matsuda I,Kataoka T
Int J Mol Sci. 2017 Dec 2;18(12):
Pubmed id:26460147 Betulinic acid and oleanolic acid, natural pentacyclic triterpenoids, interfere with N-linked glycan modifications to intercellular adhesion molecule-1, but not its intracellular transport to the cell surface.
Hiramatsu R,Fukuhara S,Mitsuda S,Yokomichi T,Kataoka T
Eur J Pharmacol. 2015 Nov 15;767():126-34
Pubmed id:25118107 Allantopyrone A, an α-pyrone metabolite from an endophytic fungus, inhibits the tumor necrosis factor α-induced nuclear factor κB signaling pathway.
Yokoigawa J,Morimoto K,Shiono Y,Uesugi S,Kimura K,Kataoka T
J Antibiot (Tokyo). 2015 Feb;68(2):71-5
Pubmed id:25627015 Irciniastatin A, a pederin-type translation inhibitor, promotes ectodomain shedding of cell-surface tumor necrosis factor receptor 1.
Hirano S,Quach HT,Watanabe T,Kanoh N,Iwabuchi Y,Usui T,Kataoka T
J Antibiot (Tokyo). 2015 Jun;68(6):417-20
Pubmed id:26027837 Irciniastatin A induces potent and sustained activation of extracellular signal-regulated kinase and thereby promotes ectodomain shedding of tumor necrosis factor receptor 1 in human lung carcinoma A549 cells.
Quach HT,Hirano S,Fukuhara S,Watanabe T,Kanoh N,Iwabuchi Y,Usui T,Kataoka T
Biol Pharm Bull. 2015;38(6):941-6
Pubmed id:25744456 Cardenolide aglycones inhibit tumor necrosis factor α-induced expression of intercellular adhesion molecule-1 at the translation step by blocking Na⁺/K⁺-ATPase.
Okina Y,Takeuchi F,Yokomichi T,Takada Y,Kataoka T
Biol Pharm Bull. 2015;38(1):39-47
Pubmed id:24649404 Ursolic acid, a natural pentacyclic triterpenoid, inhibits intracellular trafficking of proteins and induces accumulation of intercellular adhesion molecule-1 linked to high-mannose-type glycans in the endoplasmic reticulum.
Mitsuda S,Yokomichi T,Yokoigawa J,Kataoka T
FEBS Open Bio. 2014;4():229-39
Pubmed id:23357557 Deoxynivalenol induces ectodomain shedding of TNF receptor 1 and thereby inhibits the TNF-α-induced NF-κB signaling pathway.
Hirano S,Kataoka T
Eur J Pharmacol. 2013 Feb 15;701(1-3):144-51
Pubmed id:22153345 Eudesmane-type sesquiterpene lactones inhibit multiple steps in the NF-κB signaling pathway induced by inflammatory cytokines.
Tamura R,Chen Y,Shinozaki M,Arao K,Wang L,Tang W,Hirano S,Ogura H,Mitsui T,Taketani S,Ando M,Kataoka T
Bioorg Med Chem Lett. 2012 Jan 1;22(1):207-11
Pubmed id:24970122 Ursolic Acid Inhibits Na+/K+-ATPase Activity and Prevents TNF-α-Induced Gene Expression by Blocking Amino Acid Transport and Cellular Protein Synthesis.
Yokomichi T,Morimoto K,Oshima N,Yamada Y,Fu L,Taketani S,Ando M,Kataoka T
Biomolecules. 2011 Nov 7;1(1):32-47
Pubmed id:28966239

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LOT Information

Cell No.   JCRB0076 Cell Name   
LOT No.   12122011 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 0.1 mM non-essential amino acids and 10% fetal bovine serum (FBS, GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   5 - 7 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Passage twice a week.
Doubling Time   approx. 24 hrs. Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   97.9 Antibiotics Used   free
Passage Number   P45 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0076 Cell Name   
LOT No.   07022009 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 0.1 mM non-essential amino acids and 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   3 - 10 x 10^4 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 20 hrs Cell Number in Vial (cells/1ml)   2.5 x 10^6
Viability at cell freezing (%)   99.2 Antibiotics Used   free
Passage Number   P45 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0076 Cell Name   
LOT No.   07262006 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   0.7 - 1.5 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   27 hrs Cell Number in Vial (cells/1ml)   3.4 x 10^6
Viability at cell freezing (%)   97 Antibiotics Used   free
Passage Number   P45 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0076 Cell Name   
LOT No.   07292004 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   5 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   ca. 17 hrs Cell Number in Vial (cells/1ml)   4.8 x 10^6
Viability at cell freezing (%)   91.5 Antibiotics Used   free
Passage Number   P44 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0076 Cell Name   A549
LOT No.   101496 Lot Specification   distribution
Medium    Temperature   
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0076 Cell Name   A549
LOT No.   101193 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids and 10%fetal bovine serum/JRSDO10400 Temperature   37 C
Cell Density at Seeding   2x10^4 cells/sq.cm. Methods for Passages   Cells are treated with 0.05% trypsin and 0.02% EDTA
Doubling Time   18.7 hrs(graph) Cell Number in Vial (cells/1ml)   5.20x10^6
Viability at cell freezing (%)   95.4 Antibiotics Used   free
Passage Number   P41 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0076 Cell Name   A549
LOT No.   021891 Lot Specification   distribution
Medium   MEM w/ non essential amino acids + FCS 10% Temperature   37 C
Cell Density at Seeding   dilution 1/4 - 1/20 Methods for Passages   0.02% EDTA and 0.25% Trypsin
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.0x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P42 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0076 Cell Name   A549
LOT No.   083189 Lot Specification   distribution
Medium   MEM w/ non essential amino acids + FCS 10% Temperature   37
Cell Density at Seeding   dilution 1/4 - 1/20 Methods for Passages   0.02% EDTA and 0.25% Trypsin
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.7x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P41 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0076 Cell Name   A549
LOT No.   061187 Lot Specification   distribution
Medium   MEM w/ non essential amino acids + FCS 10% Temperature   37
Cell Density at Seeding   dilution 1/4 - 1/20 Methods for Passages   0.02% EDTA and 0.25% Trypsin
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.1x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P40 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0076 Cell Name   A549
LOT No.   021892 Lot Specification   distribution
Medium   MEM with NEAA + FCS 10% Temperature   37 C
Cell Density at Seeding   dilution 1/4 - 1/20 Methods for Passages   0.02 % EDTA + 0.25 % trypsin
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.2x10^6
Viability at cell freezing (%)   94.1 Antibiotics Used   free
Passage Number   P39 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion    Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0076 Cell Name   
LOT No.   083096 Lot Specification   distribution
Medium   Eagle's MEM with non-essential amino acids and 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   2-8 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   2.6 x 10^6
Viability at cell freezing (%)   92.0 Antibiotics Used   free
Passage Number   P41 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human by NP, G6PD (type B), AST, PEPB
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0076 Cell Name   A549
LOT No.   05192017 Lot Specification   distribution
Medium   MEM(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) and NEAA(GIBCO) Temperature   37 C
Cell Density at Seeding   1.72-2.8x10^4 cells/sq.cm. Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.10x10^6
Viability at cell freezing (%)   99.24 Antibiotics Used   free
Passage Number   p44 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11
D13S317:11
D7S820:8,11
D16S539:11,12
VWA:14
TH01:8,9.3
AM:X,Y
TPOX:8,11
CSF1PO:10,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   NT
Additional information   
Images
Cell No.   JCRB0076 Cell Name   A549
LOT No.   05012017 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   5.0 - 6.2 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 17 hrs. Cell Number in Vial (cells/1ml)   1.3 x 10^6
Viability at cell freezing (%)   96.3 Antibiotics Used   free
Passage Number   P44 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM with NEAA CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   96.3
Additional information   
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