JCRB0085 HL60

Cell information

Important Notice(s)

HL60細胞の培養のコツ

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0085 Cell Name   HL60
Profile   Premyoblastic cell line differentiated to neutrophils or macropahges by tumor promoters, vitamne D3 or cytekines. The cell line can be used for the research of the differenciation induction or analysis of some oncogenes. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   36
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   acute promyelotic leukemia Metastasis   
Tissue Metastasized    Genetics   APL
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   DMSO,butyl.,HX.,TPA,act.D,& R.A. ind. differentiat
Classify   tumor Established by   Collins,S.J. et al.
Registered by   Hozumi,M. Regulation for Distribution   free
Comment   Doubling time is 40hr. Growth is not so good from thawing to 1 month. Inoculate about 10^6 cells to 60mm dish when thawing. STR-PCR indicated the HL60 is same as HL60(S) (JCRB0163), Year   1986
Medium   RPMI1640 medium with 20% fetal calf serum. Methods for Passages   Simple dilution.
Cell Number on Passage    Race   Cocasian
CO2 Conc.   5 % Tissue Sampling   peripheral blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0002
Reference
Pubmed id:10470292Contribution of the nucleoside transport system to doxorubicin transport in HL60 cells but not in mononuclear cells.
Nagasawa K,Fumihara T,Ohnishi N,Yokoyama T
Jpn J Cancer Res. 1999 Jul;90(7):781-7
Pubmed id:6192693Fundamentals of chemotherapy of myeloid leukemia by induction of leukemia cell differentiation.
Hozumi M
Adv Cancer Res. 1983;38():121-69
Pubmed id:288488Characterization of the continuous, differentiating myeloid cell line (HL-60) from a patient with acute promyelocytic leukemia.
Gallagher R,Collins S,Trujillo J,McCredie K,Ahearn M,Tsai S,Metzgar R,Aulakh G,Ting R,Ruscetti F,Gallo R
Blood. 1979 Sep;54(3):713-33
Pubmed id:271272Continuous growth and differentiation of human myeloid leukaemic cells in suspension culture.
Collins SJ,Gallo RC,Gallagher RE
Nature. 1977 Nov 24;270(5635):347-9

Images



LOT Information

Cell No.   JCRB0085 Cell Name   HL60
LOT No.   08192010 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (GIBCO 10099). Use 20% FBS until cell growth becomes log phase. Temperature   37 C
Cell Density at Seeding   1 - 3 x 10^5 cells/ml Methods for Passages   dilution (解凍時はフラスコを傾けて細胞を角に集めて培養すると増殖の立ち上がりが早いです)
Doubling Time    Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   79 Antibiotics Used   free
Passage Number   Unknown (10 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   79
Additional information   
Cell No.   JCRB0085 Cell Name   
LOT No.   04282009 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (GIBCO 10099). Use 20% FBS until cell growth becomes log phase. Temperature   37 C
Cell Density at Seeding   1 - 3 x 10^5 cells/ml Methods for Passages   dilution (解凍時はフラスコを傾けて細胞を角に集めて培養すると増殖の立ち上がりが早いです)
Doubling Time    Cell Number in Vial (cells/1ml)   4.8 x 10^6
Viability at cell freezing (%)   92.5 Antibiotics Used   free
Passage Number   Unknown (12 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0085 Cell Name   
LOT No.   11182005 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 4218333S). Use 20% FBS until cells growth becomes log phase. Temperature   37 C
Cell Density at Seeding   1.5 - 3 x 10^5 cells/ml. Methods for Passages   dilution (解凍時はフラスコを傾けて細胞を角に集めて培養すると増殖の立ち上がりが早いです)
Doubling Time   30-40 hrs Cell Number in Vial (cells/1ml)   9.3 x 10^5
Viability at cell freezing (%)   92 Antibiotics Used   free
Passage Number   Unknown (12 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0085 Cell Name   
LOT No.   11062001 Lot Specification   distribution
Medium   RPMI1640 medium with 20% fetal bovine serum(非働化を推奨。対数増殖期は10%で培養可能) Temperature   37 C
Cell Density at Seeding   1.5-3.0 x 10^5 cells/ml Methods for Passages   dilution. 解凍直後は5mlの培地, FBS 20%, フラスコを傾けて培養すると立上りが良好
Doubling Time   D.T. = ca 40 hrs Cell Number in Vial (cells/1ml)   3.0 x 10^6
Viability at cell freezing (%)   87.0 Antibiotics Used   free
Passage Number   P12* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS-RPMI1640 CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0085 Cell Name   HL60
LOT No.   042197 Lot Specification   distribution
Medium   RPMI1640 medium with 10% or 20% heat inactivated fetal bovine serum (Cell Culture T.1604309). Temperature   37 C
Cell Density at Seeding   2x10^5 cells/ml Methods for Passages   Simple dilution, because of a suspension culture.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.93x10^6
Viability at cell freezing (%)   96.3 Antibiotics Used   free
Passage Number   P10* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   Under investigation.
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0085 Cell Name   HL60
LOT No.   051496 Lot Specification   distribution
Medium    Temperature   
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0085 Cell Name   HL60
LOT No.   031495 Lot Specification   distribution
Medium   RPMI1640 medium with 20% heat inactivated fetal bovine serum (JRS.DO10400) Temperature   37 C
Cell Density at Seeding   2-5x10^5 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.9x10^6
Viability at cell freezing (%)   92.8 Antibiotics Used   free
Passage Number   P7* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0085 Cell Name   HL60
LOT No.   073093 Lot Specification   distribution
Medium   RPMI1640 medium with 20% fetal bovine serum (Hyclone lot.1151031) Temperature   37 C
Cell Density at Seeding   2-5x10^5 cells/ml Methods for Passages   Cells are simply diluted with fresh medium.
Doubling Time   86.6hr->51.4hr Cell Number in Vial (cells/1ml)   3.6x10^6
Viability at cell freezing (%)   81.3 Antibiotics Used   free
Passage Number   P13* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LDH,G6PD,NP G6PD=type B
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0085 Cell Name   HL60
LOT No.   080493 Lot Specification   distribution
Medium   RPMI1640 medium with 20% fetal bovine serum (JRH Biosciences Lot.1B1062) Temperature   37 C
Cell Density at Seeding   2-3x10^5 cells/ml Methods for Passages   Cells are simply diluted by the fresh medium.
Doubling Time   Td=45.2-52.2hrs Cell Number in Vial (cells/1ml)   4.3x10^6
Viability at cell freezing (%)   78.3 Antibiotics Used   free
Passage Number   P14* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0085 Cell Name   HL60
LOT No.   080593 Lot Specification   distribution
Medium   RPMI1640 medium with 20% fetal bovine serum (JRH Biosciences, lot.1B1062) Temperature   37 C
Cell Density at Seeding   2-3x10^5 cells/ml Methods for Passages   Cells are diluted with fresh medium.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.8x10^6
Viability at cell freezing (%)   84.5 Antibiotics Used   free
Passage Number   P14* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0085 Cell Name   HL60
LOT No.   091792 Lot Specification   distribution
Medium   RPMI1640 medium (081792) with 10% fetal bovine serum (Hyclone 11151031) Temperature   37 C
Cell Density at Seeding   1.1-1.5x10^5 cells/ml Methods for Passages   Simple dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.37x10^6
Viability at cell freezing (%)   94.9 Antibiotics Used   free
Passage Number   P7* =correct. PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   M=46 Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0085 Cell Name   HL60
LOT No.   042092 Lot Specification   distribution
Medium   RPMI1640(lot.021992,041592)+10% FBS(lot.Cell Cult.Lab. 0099-01) Temperature   37 C
Cell Density at Seeding   3x10^4 cells/sq.cm Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   8.9x10^6
Viability at cell freezing (%)   86.9 Antibiotics Used   free
Passage Number   P7* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0085 Cell Name   HL60
LOT No.   062091 Lot Specification   distribution
Medium   RPMI1640 + 10 % FBS Temperature   37
Cell Density at Seeding   1-2x10^5 cells/ml Methods for Passages   Dilution subculture
Doubling Time   NT Cell Number in Vial (cells/1ml)   1x10^6
Viability at cell freezing (%)   97.4 Antibiotics Used   free
Passage Number   P6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0085 Cell Name   HL60
LOT No.   090790 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   1-2x10^5 cells/ml Methods for Passages   Dilute by fresh medium, and subculture every 6-7 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   5.0x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P7 PDL   
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0085 Cell Name   HL60
LOT No.   051590 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   1-2x10^5 cells/ml Methods for Passages   Dilute by fresh medium, and subculture every 6-7 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.5x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P8 PDL   
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0085 Cell Name   HL60
LOT No.   101189 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   1-2x10^5 cells/ml Methods for Passages   Dilute by fresh medium, and subculture every 6-7 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.7x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P7* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0085 Cell Name   HL60
LOT No.   080687 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   1-2x10^5 cells/ml Methods for Passages   Dilute by fresh medium, and subculture every 6-7 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   9x10^5
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P6* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0085 Cell Name   
LOT No.   043097 Lot Specification   distribution
Medium   RPMI1640 with 20% fetal bovine serum(FBSは熱非働化を推奨)。対数増殖期にはFBS10%にて培養可能。 Temperature   37 C
Cell Density at Seeding   ca 2 x 10^5 cells/ml Methods for Passages   希釈。解凍時は5mlの培地から御培養下さい。フラスコ を傾け細胞を寄集めた状態で培養すると立上りが良好。
Doubling Time    Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability at cell freezing (%)   93.0 Antibiotics Used   free
Passage Number   P13* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0085 Cell Name   HL60
LOT No.   02082016 Lot Specification   distribution
Medium   RPMI1640 medium with 20% fetal bovine serum (FBS; GIBCO Cat. # 10091). Temperature   37 C
Cell Density at Seeding   2.6 - 2.8 x 10^5 cells/mL Methods for Passages   Simple dilution. (Slant culture to achieve local high cell density on first seeding)
Doubling Time   approx. 31 hrs. Cell Number in Vial (cells/1ml)   3.6 x 10^6
Viability at cell freezing (%)   84 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   84
Additional information   
Cell No.   JCRB0085 Cell Name   HL60
LOT No.   02152018 Lot Specification   distribution
Medium   RPMI1640 medium with 20% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   0.7 - 1.3 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture). (解凍時はフラスコを傾けて細胞を角に集めて培養すると増殖の立ち上がりが早いです To improve first growth, the flask should be slant slightly to gather cells to the corner. )
Doubling Time   approx. 27 hrs. Cell Number in Vial (cells/1ml)   4.9 x 10^6
Viability at cell freezing (%)   90.1 Antibiotics Used   free
Passage Number   Unknown (15 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   90.1
Additional information   
Cell No.   JCRB0085 Cell Name   HL60
LOT No.   04092019 Lot Specification   distribution
Medium   RPMI 1640 medium with 20% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   approx. 1.5 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture). (解凍時はフラスコを傾けて細胞を角に集めて培養すると増殖の立ち上がりが早いです To improve first growth, the flask should be slant slightly to gather cells to the corner. )
Doubling Time   approx. 32 hrs. Cell Number in Vial (cells/1ml)   2.8 x 10^6
Viability at cell freezing (%)   91.7 Antibiotics Used   free
Passage Number   Unknown (10 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   83.0
Additional information   
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