JCRB0091 P31/FUJ

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0091 Cell Name   P31/FUJ
Profile   leukemia, acute monocytic Other Name   Fujioka
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   acute monocytic leukemia Metastasis   
Tissue Metastasized    Genetics   monocyte, 47:XY, Dt=80hr
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   Pox+,PAS+,AcPase+,al-pase+,NACE+,aNBE+,NaF+,Pago-
Classify   tumor Established by   Hirose,M.
Registered by   Minato,K. Regulation for Distribution   No commercial purpose. Permission required.
Comment   Lot of FCS affects critically to growth of the cells. Year   1986
Medium   RPMI1640 medium with 10% fetal calf serum. Methods for Passages   Dilution 1/5 every 3-4 days.
Cell Number on Passage   split ratio=1/5 Race   Japanese
CO2 Conc.   5 % Tissue Sampling   peripheral blood
Virus Summary   tested Virus Tested   CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-) [-/negative, +/positive, nt/not tested]
Tissue Type   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1632
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 909253
Reference
Pubmed id:6962783 A novel monocytoid cultured cell line, P31/Fujioka, derived from acute monoblastic leukemia.
Hirose M,Minato K,Tobinai K,Shimoyama M,Watanabe S,Abe T
Gan. 1982 Oct;73(5):735-41

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LOT Information

Cell No.   JCRB0091 Cell Name   P31/FUJ
LOT No.   101388 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   dilution 1/5 Methods for Passages   Dilution 1/5 every 3-4 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.0x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P13* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0091 Cell Name   P31/FUJ
LOT No.   101186 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   dilution 1/5 Methods for Passages   Dilution 1/5 every 3-4 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   9x10^5
Viability at cell freezing (%)   79% Antibiotics Used   free
Passage Number   P8* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   D5S818:11
D13S317:9,12,13
D7S820:10
D16S539:9,10
VWA:19,21
TH01:9
AM:XY
TPOX:7,11
CSF1PO:13,14
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0091 Cell Name   
LOT No.   032497 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   1.5 x 10^5 cells/ml Methods for Passages   Dilution
Doubling Time    Cell Number in Vial (cells/1ml)   5.2 x 10^6
Viability at cell freezing (%)   96.0 Antibiotics Used   free
Passage Number   P5* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0091 Cell Name   P31/FUJ
LOT No.   03012019 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   approx. 2 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture). The size of cells is small, therefore the centrifugation at 1200 rpm for 5 min will be suitable.
Doubling Time    Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   83.5 Antibiotics Used   free
Passage Number   Unknown (4 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   71.3
Additional information   
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