JCRB0092 P39/TSU
Cell information
Important Notice(s)JCRB0092 P39/TSU is derivative from HL-60.
| JCRB No. | JCRB0092 | Cell Name | P39/TSU |
|---|---|---|---|
| Profile | Stop providing for the issue of quality control. (Please contact us for more information) Human acute myeloblastic leukemia cell line. STR-PCR data indicated that this cell line is the same cell line with the HL60. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 69 |
| Identity | available | Tissue for Primary Cancer | hemo-lymphocytic |
| Case history | acute myeloblastic leukemia | Metastasis | |
| Tissue Metastasized | Genetics | Pox-,PAS+,AcPase+,aNCAE+,aNBE+,NAF+ | |
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | tlansplantable to nude mouse,CD4+,CDw13+,CDw14+ |
| Classify | tumor | Established by | Nagai,M. |
| Registered by | Minato,K. | Regulation for Distribution | Permission required. No commercial purpose. (DNA profiling:same pattern with HL60 |
| Comment | This cell was established from patient who changed from Myelodysplastic syndrome to overt leukemia.The cell line was FAB type M2 initially but monocyte later. STR-PCR results indicated that this cell line is possibly cross contaminated with the HL60. | Year | 1986 |
| Medium | RPMI1640 medium with 10% fetal calf serum | Methods for Passages | Dilute 1/5 every 3-4 days. Lot of FCS critically affect to the cell growth. |
| Cell Number on Passage | split ratio=1/5 | Race | Japanese |
| CO2 Conc. | 5 % | Tissue Sampling | peripheral blood |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | + | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | NT | ||||||
| Notes | EBV(low copy) | ||||||||
| Images |
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LOT Information
| Cell No. | JCRB0092 | Cell Name | P39/TSU |
|---|---|---|---|
| LOT No. | 031395 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 15% heat inactivated fetal bovine serum (JRHBiosciences, 1B1062) | Temperature | 37 C |
| Cell Density at Seeding | 1-4x10^5 cells/ml | Methods for Passages | simple dilution |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 6.2x10^6 |
| Viability at cell freezing (%) | 93.7 | Antibiotics Used | free |
| Passage Number | P15* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11,12 D13S317:8,11 D7S820:11,12 D16S539:11 VWA:15,16 TH01:7,8 AM:X TPOX:8,11 CSF1PO:13,14 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Images |
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| Cell No. | JCRB0092 | Cell Name | P39/TSU |
|---|---|---|---|
| LOT No. | 102888 | Lot Specification | distribution |
| Medium | RPMI1640 + FCS 15% | Temperature | 37 |
| Cell Density at Seeding | dilution 1/5 | Methods for Passages | Dilute 1/5 every 3-4 days. Lot of FCS critically affect to the cell growth. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 3.3x10^6 |
| Viability at cell freezing (%) | NT | Antibiotics Used | free |
| Passage Number | P14* | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NP |
| Chromosome Mode | Chromosome Information | NT | |
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |