JCRB0101 A3/KAW

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0101 Cell Name   A3/KAW
Profile   lymphoma, malignant Other Name   A3
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   68
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   stomach cancer, malignant lymphoma Metastasis   
Tissue Metastasized    Genetics   CD4+,Ia-,EBNA-
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   Dt=20 hr
Classify   tumor Established by   Minato,K.
Registered by   Minato,K. Regulation for Distribution   No commercial purpose. Permission required.
Comment   Mycoplasma was eliminated by Oku, T at Dec. 1987. Year   1986
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   Dilution every 4 days.
Cell Number on Passage   10^5 cells/60mm dish Race   Japanese
CO2 Conc.   5 % Tissue Sampling   ascites
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1062
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 910935
Reference
Pubmed id:1993551Identification and characterization of a colon-cancer-associated antigen expressed on leukemia-lymphoma cell lines.
Chimori Y,Minowada J
Int J Cancer. 1991 Feb 1;47(3):426-30
Pubmed id:3981816Time-dependent cytotoxic action of human recombinant alpha-interferon (Ro22-8181) in vitro and the sensitivity of various cultured leukemia and lymphoma cell lines to it.
Shibata T,Shimada Y,Shimoyama M
Jpn J Clin Oncol. 1985 Mar;15(1):67-75
Pubmed id:6526223Growth-inhibitory activity of human recombinant beta-interferon (GKT-beta) in vitro.
Shimada Y,Shimoyama M
Gan. 1984 Dec;75(12):1116-24
Pubmed id:6601597Two novel cultured cell lines, A3/Kawakami and A4/Fukuda, derived from malignant lymphoma of B(non-T)-cell nature of the gastrointestinal tract.
Hirose M,Minato K,Tobinai K,Shimoyama M,Watanabe S,Abe T,Deura K
Gan. 1983 Feb;74(1):106-15

Images



LOT Information

Cell No.   JCRB0101 Cell Name   A3/KAW
LOT No.   01142009 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099, lot 484375) Temperature   37 C
Cell Density at Seeding   2 - 4 x 10^5 cells/ml Methods for Passages   Dilution
Doubling Time   38 hrs Cell Number in Vial (cells/1ml)   5.9 x 10^5 (viable)
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0101 Cell Name   A3/KAW
LOT No.   072189 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum. Temperature   37 C
Cell Density at Seeding   10^5 cells/6 cm dish Methods for Passages   Simple dilution every 4 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.7x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P8* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0101 Cell Name   A3/KAW
LOT No.   03142016 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   3.7 - 5.0 x 10^5 cells/mL Methods for Passages   Dilution (passage every 4 days).
Doubling Time   approx. 39 hrs Cell Number in Vial (cells/1ml)   3.6 x 10^6
Viability at cell freezing (%)   83 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   83
Additional information   
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