JCRB0102 RERF-LC-FM

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0102 Cell Name   RERF-LC-FM
Profile   small cell carcinoma, intermediate Other Name   Fujimoto
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   lung (cancer)
Case history   intermediate type, lung small cell carcinoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   create clusters on growth
Classify   tumor Established by   Akiyama,M.
Registered by   Akiyama,M. Regulation for Distribution   free
Comment    Year   1986
Medium   RPMI1640 w/ non essential amino acids + FCS 10% Methods for Passages   Dilution.
Cell Number on Passage   split ratio=1/2 - 1/3 Race   Japanese
CO2 Conc.   5 % Tissue Sampling   pleural effusion
Virus Summary   tested Virus Tested   GAPDH(+), CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), HBV(-), parvoB19(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-)[-/negative, +/positive, nt/not tested,GAPDH is positive control]
Tissue Type   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1653
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 930081
Reference
Pubmed id:4005855 Monoclonal antibodies to human squamous cell carcinoma of the lung and their application to tumor diagnosis.
Kyoizumi S,Akiyama M,Kouno N,Kobuke K,Hakoda M,Jones SL,Yamakido M
Cancer Res. 1985 Jul;45(7):3274-81

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LOT Information

Cell No.   JCRB0102 Cell Name   RERF-LC-FM
LOT No.   071991 Lot Specification   distribution
Medium   RPMI1640 w/ non essential amino acids + FCS 10% Temperature   37 C
Cell Density at Seeding   split ratio:1/2 - 1/3 Methods for Passages   Dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.2x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P16 PDL   
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0102 Cell Name   RERF-LC-FM
LOT No.   020690 Lot Specification   distribution
Medium   RPMI1640 w/ non essential amino acids + FCS 10% Temperature   37
Cell Density at Seeding   split ratio:1/2 - 1/3 Methods for Passages   Dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.6x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P13 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0102 Cell Name   RERF-LC-FM
LOT No.   101886 Lot Specification   distribution
Medium   RPMI1640 w/ non essential amino acids + FCS 10% Temperature   37
Cell Density at Seeding   split ratio:1/2 - 1/3 Methods for Passages   Dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   7x10^5
Viability at cell freezing (%)   45% Antibiotics Used   free
Passage Number   P12 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0102 Cell Name   
LOT No.   101698 Lot Specification   distribution
Medium   RPMI1940 medium with non-essential amino acids and10% fetal calf serum (FCS lot;BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   1 x 10^5 cells/ml Methods for Passages   Dilution
Doubling Time   60 hrs Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   82.0 Antibiotics Used   free
Passage Number   P14 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
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