JCRB0104 KU812

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0104 Cell Name   KU812
Profile   leukemia, chronic myeloid Other Name   KU812
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   38
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   chronic myeloid leukemia (CML) Metastasis   
Tissue Metastasized    Genetics   Ph1 chr., histamin(+),t(9;22)(q34;q11),i(17q) etc.
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   Dt=20-30hr, colony formation on soft agar=30-50%
Classify   tumor Established by   Kishi,K.
Registered by   Kishi,K. Regulation for Distribution   free
Comment   Ph1 chromosome (+). Histamine (+). This cell line was deposited to the ATCC, ECACC and DSM at 1990. Year   1987
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   Dilution.
Cell Number on Passage   10^5 cells/ml Race   Japanese
CO2 Conc.   5 % Tissue Sampling   peripheral blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0379
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 907311
Reference
Pubmed id:2540861 KU 812: a pluripotent human cell line with spontaneous erythroid terminal maturation.
Nakazawa M,Mitjavila MT,Debili N,Casadevall N,Mayeux P,Rouyer-Fessard P,Dubart A,Roméo PH,Beuzard Y,Kishi K
Blood. 1989 May 15;73(7):2003-13
Pubmed id:3695564 Induction of differentiation of the human leukemia cell line, KU812.
Fischkoff SA,Kishi K,Benjamin WR,Rossi RM,Hoessly MC,Hoxie JA,Seals C,Anderson L,Ishizaka T,Hakimi J
Leuk Res. 1987;11(12):1105-13
Pubmed id:2436686 A monoclonal antibody reacting with human basophils.
Bodger MP,Mounsey GL,Nelson J,Fitzgerald PH
Blood. 1987 May;69(5):1414-8
Pubmed id:3858609 A new leukemia cell line with Philadelphia chromosome characterized as basophil precursors.
Kishi K
Leuk Res. 1985;9(3):381-90

Images



LOT Information

Cell No.   JCRB0104 Cell Name   KU812
LOT No.   08302010 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   1.11-2.30x10^5 cells/ml Methods for Passages   Dilution,(10^5 cells/ml )
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.97x10^6
Viability at cell freezing (%)   94.2 Antibiotics Used   free
Passage Number   p5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10
D13S317:8
D7S820:12
D16S539:9
VWA:15
TH01:9
AM:X,Y
TPOX:12
CSF1PO:12,13
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0104 Cell Name   
LOT No.   07222005 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   approx. 10^5 cells/ml Methods for Passages   Dilution
Doubling Time   approx. 30 hrs Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   99.3 Antibiotics Used   free
Passage Number   Unknown (P6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0104 Cell Name   KU812
LOT No.   083093 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (Hyclone 11151031) Temperature   37 C
Cell Density at Seeding   1-2.0x10^5 cells/ml Methods for Passages   Dilution.
Doubling Time   28.1hrs. Cell Number in Vial (cells/1ml)   4.32x10^6
Viability at cell freezing (%)   92.9 Antibiotics Used   free
Passage Number   P6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, LD, PepB
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10
D13S317:8
D7S820:12
D16S539:9
VWA:15
TH01:9
AM:XY
TPOX:12
CSF1PO:12,13
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0104 Cell Name   KU812
LOT No.   082290 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   10^5 cells/ml Methods for Passages   Dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.3x10^6
Viability at cell freezing (%)   76% Antibiotics Used   free
Passage Number   P7* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0104 Cell Name   KU812
LOT No.   050690 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37 C
Cell Density at Seeding   10^5 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.4 x 10^6
Viability at cell freezing (%)   91% Antibiotics Used   free
Passage Number   P9* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0104 Cell Name   KU812
LOT No.   040290 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   10^5 cells/ml Methods for Passages   Dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.5x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P9* PDL   
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0104 Cell Name   KU812
LOT No.   082589 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   10^5 cells/ml Methods for Passages   Dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.6x10^6
Viability at cell freezing (%)   97% Antibiotics Used   free
Passage Number   P5* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0104 Cell Name   KU812
LOT No.   082187 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   10^5 cells/ml Methods for Passages   Dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   6x10^5
Viability at cell freezing (%)   96% Antibiotics Used   free
Passage Number   P4* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0104 Cell Name   KU812
LOT No.   09182015 Lot Specification   distribution
Medium   RPMI-1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   10^5 cells/mL Methods for Passages   Dilution (suspension culture)
Doubling Time    Cell Number in Vial (cells/1ml)   4.2 x 10^6
Viability at cell freezing (%)   91 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   91
Additional information   
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