JCRB0105 KY821

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0105 Cell Name   KY821
Profile   leukemia, acute myeloid Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   28
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   leukemia Metastasis   
Tissue Metastasized    Genetics   Double minute chromosome positive
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   Dt=27-37 hr
Classify   tumor Established by   Kishi,K.
Registered by   Kishi,k. Regulation for Distribution   not defined
Comment    Year   1987
Medium   RPMI1640 medium with 10% calf serum Methods for Passages   Dilution.
Cell Number on Passage    Race   Japanese
CO2 Conc.   5 % Tissue Sampling   peripheral blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1346
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 907300
Reference
Pubmed id:2112525A simple, general method for detecting retroviral RNAs expressed in cells.
Harada F,Hirose Y,Tanaka M,Sasaki T,Kamiya H,Miura K
Jpn J Cancer Res. 1990 Mar;81(3):232-7
Pubmed id:2698048Expression of cytokine genes in hematological malignancies.
Okamura S,Hayashi S,Kawasaki C,Kondo S,Asano Y,Omori F,Otsuka T,Shibuya T,Harada M,Niho Y
Nihon Ketsueki Gakkai Zasshi. 1989 Dec;52(8):1423-32

Images



LOT Information

Cell No.   JCRB0105 Cell Name   
LOT No.   11022006 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   0.5 - 2 x 10^5 cells/ml Methods for Passages   Dilution. The initiation of growth after thawing is slow.
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   98.5 Antibiotics Used   free
Passage Number   P5 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD and LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0105 Cell Name   KY821
LOT No.   112588 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding    Methods for Passages   Dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.1x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P6* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0105 Cell Name   KY821
LOT No.   08062018 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   2.1-4.1x10^5 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.1x10^6
Viability at cell freezing (%)   98.70 Antibiotics Used   free
Passage Number   p9* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,13
D13S317:9,12
D7S820:12
D16S539:10,12
VWA:17,18
TH01:9
AM:X
TPOX:11
CSF1PO:10,12
Adhesion   No Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   NT Additional information   
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