JCRB0112 THP-1

Cell information

Important Notice(s)

THP-1細胞の登録番号JCRB0112, JCRB0112.1の違い / Difference in JCRB0112 and JCRB0112.1

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0112 Cell Name   THP-1
Profile   Human acute monocytic leukemia. Other Name   THP-1
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   young
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   acute monocytic leukemia Metastasis   No
Tissue Metastasized    Genetics   Fc,C3b receptors. HLA-A2,-A9,-B5,-DRW1, & -DRW2.
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   No immunoglobulins produced. No EBV.
Classify   tumor Established by   Tsuchiya,S.
Registered by   Tsuchiya, S. Regulation for Distribution   no regulation applied
Comment   HLA-DR,My 7, My 9, OKM1 positive. Nonspecific esterase positive, Peroxidase negative, a-naftol ASD chloroacetate esterase negative, etc. by Dr. Tsuchiya. Year   1987
Medium   RPMI1640 medium with 5% fetal calf serum Methods for Passages   Dilution, twice a week.
Cell Number on Passage   2-5x10^5 cells/ml Race   Japanese
CO2 Conc.   5 % Tissue Sampling   peripheral blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0006
Reference
Pubmed id:2964957Regulation of Fc epsilon receptor expression on a human monoblast cell line U937.
Mayumi M,Kawabe T,Kim KM,Heike T,Katamura K,Yodoi J,Mikawa H
Clin Exp Immunol. 1988 Jan;71(1):202-6
Pubmed id:3100613Lymphotoxin: induction of terminal differentiation of the human myeloid leukemia cell lines HL-60 and THP-1.
Hemmi H,Nakamura T,Tamura K,Shimizu Y,Kato S,Miki T,Takahashi N,Muramatsu M,Numao N,Sugamura K
J Immunol. 1987 Feb 1;138(3):664-6
Pubmed id:2989063Induction of functional differentiation of a human monocytic leukemia cell line (THP-1) by retinoic acid and cholera toxin.
Hemmi H,Breitman TR
Jpn J Cancer Res. 1985 May;76(5):345-51
Pubmed id:3855620Cells of a human monocytic leukemia cell line (THP-1) synthesize and secrete apolipoprotein E and lipoprotein lipase.
Tajima S,Hayashi R,Tsuchiya S,Miyake Y,Yamamoto A
Biochem Biophys Res Commun. 1985 Jan 16;126(1):526-31
Pubmed id:6091813Lineage infidelity of a human myelogenous leukemia cell line.
Palumbo A,Minowada J,Erikson J,Croce CM,Rovera G
Blood. 1984 Nov;64(5):1059-63
Pubmed id:6949641Induction of maturation in cultured human monocytic leukemia cells by a phorbol diester.
Tsuchiya S,Kobayashi Y,Goto Y,Okumura H,Nakae S,Konno T,Tada K
Cancer Res. 1982 Apr;42(4):1530-6
Pubmed id:6970727Establishment and characterization of a human acute monocytic leukemia cell line (THP-1).
Tsuchiya S,Yamabe M,Yamaguchi Y,Kobayashi Y,Konno T,Tada K
Int J Cancer. 1980 Aug;26(2):171-6

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LOT Information

Cell No.   JCRB0112 Cell Name   THP-1
LOT No.   07122011 Lot Specification   distribution
Medium   RPMI 1640 medium with 5% fetal bovine serum (GIBCO Cat. # 10099). Please increase FBS concentration to 10% several days after thawing. Temperature   37 C
Cell Density at Seeding   3.4 - 8.2 x 10^4 cells/mL Methods for Passages   Simple dilution.
Doubling Time   approx. 31 hrs. Cell Number in Vial (cells/1ml)   2.7 x 10^6
Viability at cell freezing (%)   95.1 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11,12
D13S317:8,13
D7S820:10
D16S539:11,12
VWA:16,17
TH01:8,9.3
AM:X,Y
TPOX:8,11
CSF1PO:11,13
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0112 Cell Name   THP-1
LOT No.   05252009 Lot Specification   distribution
Medium   RPMI 1640 medium with 5% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   3 - 10 x 10^4 cells/ml Methods for Passages   Dilution (suspension culture)
Doubling Time   approx. 41 hrs Cell Number in Vial (cells/1ml)   2.3 x 10^6
Viability at cell freezing (%)   78.3 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   91%
Additional information   Expected viability (defrost ampoule) 91 %.
Cell No.   JCRB0112 Cell Name   
LOT No.   11272006 Lot Specification   distribution
Medium   RPMI 1640 medium with 5% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   0.3 - 1 x 10^5 cells/ml Methods for Passages   Dilution
Doubling Time   approx. 1.5 days Cell Number in Vial (cells/1ml)   3.6 x 10^6
Viability at cell freezing (%)   98.3 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD, and LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0112 Cell Name   
LOT No.   04302002 Lot Specification   distribution
Medium   RPMI1640 medium with 5% fetal bovine serum (at thawing, use 10% FBS). FBS lot used; BioWhittaker 0S069F. Temperature   37 C
Cell Density at Seeding   5-10 x 10^4 cells/ml Methods for Passages   dilution
Doubling Time   D.T. = ca 38 hrs Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   88 Antibiotics Used   free
Passage Number   P7 at bank PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD. LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS-RPMI1640 CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0112 Cell Name   THP-1
LOT No.   080194 Lot Specification   distribution
Medium   RPMI1640 medium with 10% heat-inactivated fetal bovine serum (J.R.Scientific, DO10400, Hyc.11151031) Temperature   37 C
Cell Density at Seeding   1-3x10^5 cells/ml Methods for Passages   Simple dilution method.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.4x10^6
Viability at cell freezing (%)   96.5 Antibiotics Used   free
Passage Number   P8* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0112 Cell Name   THP-1
LOT No.   F00952 Lot Specification   distribution
Medium   RPMI1640 medium with 5% fetal bovine serum (BioWhittaker, lot.3M0581) Temperature   37 C
Cell Density at Seeding    Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.5x10^6
Viability at cell freezing (%)   82.0 Antibiotics Used   free
Passage Number   P7* PDL   NT
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0112 Cell Name   THP-1
LOT No.   092292 Lot Specification   distribution
Medium   RPMI1640 medium (081792) with 5% fetal bovine serum heat inactivated (Flow 0070893) Temperature   37 C
Cell Density at Seeding   8.5x10^4 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.79x10^6
Viability at cell freezing (%)   95.3 Antibiotics Used   free
Passage Number   P5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0112 Cell Name   THP-1
LOT No.   111391 Lot Specification   distribution
Medium   RPMI1640 + FCS 5% Temperature   37 C
Cell Density at Seeding   2-5x10^5 cells/10cm dish Methods for Passages   dilution, twice a week
Doubling Time   DT=30.3 hr Cell Number in Vial (cells/1ml)   1.1x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP,LD,G6PD
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0112 Cell Name   THP-1
LOT No.   101190 Lot Specification   distribution
Medium   RPMI1640 + FCS 5% Temperature   37
Cell Density at Seeding   2-5x10^5 cells/10cm dish Methods for Passages   Dilution, twice a week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   5.7x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P5* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0112 Cell Name   THP-1
LOT No.   061288 Lot Specification   distribution
Medium   RPMI1640 + FCS 5% Temperature   37
Cell Density at Seeding   2-5x10^5 cells/10cm dish Methods for Passages   Dilution, twice a week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   5.6x10^5
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P4* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LD,NP,G6PD
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0112 Cell Name   
LOT No.   062598 Lot Specification   distribution
Medium   RPMI1640 medium with 5% fetal calf serum (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   1 x 10^5 cells/ml Methods for Passages   Dilution
Doubling Time    Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   95.0 Antibiotics Used   free
Passage Number   P6* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0112 Cell Name   THP-1
LOT No.   51020201 Lot Specification   distribution
Medium   RPMI 1640 medium with 5% fetal bovine serum (GIBCO Cat. # 10091). Please increase FBS concentration to 10 - 20% several days after thawing. Temperature   37 C
Cell Density at Seeding   3 - 5 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time   approx. 56 hrs Cell Number in Vial (cells/1ml)   4.6 x 10^6
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   90
Additional information   
Cell No.   JCRB0112 Cell Name   THP-1
LOT No.   10192016 Lot Specification   distribution
Medium   RPMI-1640 medium with 5% fetal bovine serum (FBS; Sigma Cat. # 172012). Use 10% FBS until cells stably grow. Temperature   37 C
Cell Density at Seeding   3.7 - 8.4 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time   approx. 57 hrs. Cell Number in Vial (cells/1ml)   5.9 x 10^6
Viability at cell freezing (%)   91 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   5% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   91
Additional information   
Cell No.   JCRB0112 Cell Name   THP-1
LOT No.   02052018 Lot Specification   distribution
Medium   RPMI 1640 medium with 5% heat-inactivated fetal bovine serum (FBS; Sigma Cat. # 17012). Use 10% FBS until cells stably grow. Temperature   37 C
Cell Density at Seeding   1.1 - 3.4 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time   approx. 29 hrs. Cell Number in Vial (cells/1ml)   2.6 x 10^6
Viability at cell freezing (%)   93.9 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   5% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   NT
Additional information   
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