JCRB0112.1 THP-1

Cell information

Important Notice(s)

THP-1細胞の登録番号JCRB0112, JCRB0112.1の違い / Difference in JCRB0112 and JCRB0112.1

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0112.1 Cell Name   THP-1
Profile   Human acute monocytic leukemia. Other Name   THP-1/ATCC
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   young
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   acute monocytic leukemia Metastasis   No
Tissue Metastasized    Genetics   Fc,C3b receptor.HLA-A2,-A9,-B5,-DRW1,-DRW2.
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   No immuno globulin productions.
Classify   tumor Established by   Tsuchiya,S.
Registered by   Tsuchiya,S. Regulation for Distribution   Regulation not applied.
Comment   HLA-DR, My7, My9, OKM1 positive. Nonspecific peroxidase positive, peroxidase negative, a-naftol ASD chloroacetate esterase negative. by Dr. Tsuchiya. Year   1987
Medium   RPMI1640 medium with 5% fetal calf serum. Methods for Passages   Dilution, twice a week.
Cell Number on Passage   2-5x10^5 cells/ml Race   Japanese
CO2 Conc.   5 % Tissue Sampling   peripheral blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0006
Reference
Pubmed id:2964957Regulation of Fc epsilon receptor expression on a human monoblast cell line U937.
Mayumi M,Kawabe T,Kim KM,Heike T,Katamura K,Yodoi J,Mikawa H
Clin Exp Immunol. 1988 Jan;71(1):202-6
Pubmed id:3100613Lymphotoxin: induction of terminal differentiation of the human myeloid leukemia cell lines HL-60 and THP-1.
Hemmi H,Nakamura T,Tamura K,Shimizu Y,Kato S,Miki T,Takahashi N,Muramatsu M,Numao N,Sugamura K
J Immunol. 1987 Feb 1;138(3):664-6
Pubmed id:2989063Induction of functional differentiation of a human monocytic leukemia cell line (THP-1) by retinoic acid and cholera toxin.
Hemmi H,Breitman TR
Jpn J Cancer Res. 1985 May;76(5):345-51
Pubmed id:3855620Cells of a human monocytic leukemia cell line (THP-1) synthesize and secrete apolipoprotein E and lipoprotein lipase.
Tajima S,Hayashi R,Tsuchiya S,Miyake Y,Yamamoto A
Biochem Biophys Res Commun. 1985 Jan 16;126(1):526-31
Pubmed id:6091813Lineage infidelity of a human myelogenous leukemia cell line.
Palumbo A,Minowada J,Erikson J,Croce CM,Rovera G
Blood. 1984 Nov;64(5):1059-63
Pubmed id:6949641Induction of maturation in cultured human monocytic leukemia cells by a phorbol diester.
Tsuchiya S,Kobayashi Y,Goto Y,Okumura H,Nakae S,Konno T,Tada K
Cancer Res. 1982 Apr;42(4):1530-6
Pubmed id:6970727Establishment and characterization of a human acute monocytic leukemia cell line (THP-1).
Tsuchiya S,Yamabe M,Yamaguchi Y,Kobayashi Y,Konno T,Tada K
Int J Cancer. 1980 Aug;26(2):171-6

Images



LOT Information

Cell No.   JCRB0112.1 Cell Name   
LOT No.   06272012 Lot Specification   distribution
Medium   RPMI 1640 medium with 5% fetal bovine serum (FBS; GIBCO Cat. # 10099). Use 10% FBS until cells grow stably. Temperature   37 C
Cell Density at Seeding   1.5 - 4.0 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture)
Doubling Time   approx. 42 hrs Cell Number in Vial (cells/1ml)   2.3 x 10^6
Viability at cell freezing (%)   95.2 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0112.1 Cell Name   THP-1
LOT No.   09032010 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 5% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   0.5-1x10^5 cells/ml Methods for Passages   dilution,twice a week
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.92x10^6
Viability at cell freezing (%)   99.1 Antibiotics Used   free
Passage Number   p6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:13
D7S820:10
D16S539:11,12
VWA:16
TH01:8,9.3
AM:X,Y
TPOX:8,11
CSF1PO:11,13
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0112.1 Cell Name   
LOT No.   11212005 Lot Specification   distribution
Medium   RPMI1640 medium with 5% fetal bovine serum (FBS lot; GIBCO 4218333S). Use 10% FBS until cells grow well after thawing. Temperature   37 C
Cell Density at Seeding   1 - 1.5 x 10^5 cells/ml Methods for Passages   Dilution
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   90.8 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0112.1 Cell Name   THP-1
LOT No.   072396 Lot Specification   distribution
Medium    Temperature   
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   G6PD, LDH, NP
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0112.1 Cell Name   THP-1
LOT No.   012492 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (Cell Culture Lab.00939-01) Temperature   37C
Cell Density at Seeding   2x10^6 cells/10cm dish Methods for Passages   dilution
Doubling Time   24 hr Cell Number in Vial (cells/1ml)   4.3x10^6
Viability at cell freezing (%)   95.7 Antibiotics Used   free
Passage Number   P5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   M=49 Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0112.1 Cell Name   THP-1
LOT No.   112488 Lot Specification   distribution
Medium   RPMI1640 + FCS 5% Temperature   37
Cell Density at Seeding   2-5x10^5 cells/ml Methods for Passages   Dilution, twice a week
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.6x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P5* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0112.1 Cell Name   THP-1
LOT No.   09222014 Lot Specification   distribution
Medium   RPMI1640 medium with 5% heat-inactivated fetal bovine serum (FBS; GIBCO Cat. # 10091) use 10% FBS at the intiation of culture. Temperature   37 C
Cell Density at Seeding   2 - 5 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture)
Doubling Time    Cell Number in Vial (cells/1ml)   7.2 x 10^6
Viability at cell freezing (%)   92 Antibiotics Used   free
Passage Number   Unknown (10 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11,12
D13S317:13
D7S820:10
D16S539:11,12
VWA:16
TH01:8,9.3
AM:X,Y
TPOX:8,11
CSF1PO:11,13
Adhesion   No Exoteric Gene   
Medium for Freezing   Cell Banker 1 (Nippon Zenyaku Industries) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   92
Additional information   
Cell No.   JCRB0112.1 Cell Name   
LOT No.   050297 Lot Specification   distribution
Medium   RPMI1640 medium with 5% fetal calf serum (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   1-2 x 10^5 cells/ml Methods for Passages   Dilution
Doubling Time   approx. 42 hrs Cell Number in Vial (cells/1ml)   1.0 x 10^6
Viability at cell freezing (%)   64.0 Antibiotics Used   free
Passage Number   P7* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0112.1 Cell Name   
LOT No.   F00952 Lot Specification   distribution
Medium   RPMI1640 + 10% FCS Temperature   37
Cell Density at Seeding    Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   82.0 Antibiotics Used   free
Passage Number   * +5 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human by isoenzymology
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0112.1 Cell Name   THP-1
LOT No.   10282015 Lot Specification   distribution
Medium   RPMI1640 medium with 5% heat-inactivated fetal bovine serum (FBS; GIBCO Cat. # 10091). Use 10% FBS until cells grow stably. Temperature   37 C
Cell Density at Seeding   3.0 - 7.5 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time   66 hrs Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   86.9 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   86.9
Additional information   
Cell No.   JCRB0112.1 Cell Name   THP-1
LOT No.   11012017 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 5% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   1.19-1.21x10^5 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.04x10^6
Viability at cell freezing (%)   100 Antibiotics Used   free
Passage Number   p8* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:13
D7S820:10
D16S539:11,12
VWA:16
TH01:8,9.3
AM:X,Y
TPOX:8,11
CSF1PO:11,13
Adhesion   No Exoteric Gene   NT
Medium for Freezing   RPMI1640 medium with 5% DMSO and 20%heat inactivated fetal bovine serum CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   NT
Additional information   Use 10% heat inactivated fetal bovine serum until cells grow stably
Images
Cell No.   JCRB0112.1 Cell Name   THP-1
LOT No.   11102017 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 5% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   1.00-1.21x10^5 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.52x10^6
Viability at cell freezing (%)   100 Antibiotics Used   free
Passage Number   p10* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:13
D7S820:10
D16S539:11,12
VWA:16
TH01:8,9.3
AM:X,Y
TPOX:8,11
CSF1PO:11,13
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   NT
Additional information   Use 10% heat inactivated fetal bovine serum until cells grow stably.
Images
Cell No.   JCRB0112.1 Cell Name   THP-1
LOT No.   11092017 Lot Specification   distribution
Medium   RPMI-1640 medium with 5% fetal bovine serum (FBS; Sigma Cat. # 172012). Use 10% FBS until cells stably grow. Temperature   37 C
Cell Density at Seeding   2 - 4 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time   approx. 41 hrs. Cell Number in Vial (cells/1ml)   2.0 x 10^6
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   5% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   90
Additional information   
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