JCRB0116.1 U937 cl1-14
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0116.1 | Cell Name | U937 cl1-14 |
|---|---|---|---|
| Profile | lymphoma, histiocytic, highly susceptible clone of U937 for HIV | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | subline, U937 |
| Identity | available | Tissue for Primary Cancer | hemo-lymphocytic |
| Case history | histiocytic lymphoma | Metastasis | |
| Tissue Metastasized | Genetics | express monocyte-like characteristics | |
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | highly susceptible clone for HIV virus |
| Classify | tumor | Established by | Tabata,K. |
| Registered by | Ohnishi,K. | Regulation for Distribution | free |
| Comment | Year | 1992 | |
| Medium | RPMI1640 medium with 10% fetal calf serum | Methods for Passages | dilution |
| Cell Number on Passage | 5 x 10^4 cells/ml | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | pleural effusion |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:7381208 | Chemotactically responsive and nonresposive forms of a continuous human monocyte cell line. Fischer DG,Pike MC,Koren HS,Snyderman R J Immunol. 1980 Jul;125(1):463-5 |
| Pubmed id:1083890 | Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines. Ralph P,Moore MA,Nilsson K J Exp Med. 1976 Jun 1;143(6):1528-33 |
| Pubmed id:178611 | Establishment and characterization of a human histiocytic lymphoma cell line (U-937). Sundström C,Nilsson K Int J Cancer. 1976 May 15;17(5):565-77 |
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LOT Information
| Cell No. | JCRB0116.1 | Cell Name | U937 cl1-14 |
|---|---|---|---|
| LOT No. | 012194 | Lot Specification | distribution |
| Medium | RPMI1648 medium with 10% heat inactivated fetal bovine serum | Temperature | 37 C |
| Cell Density at Seeding | 1x10^5 cells/ml | Methods for Passages | Simple dilution. Dilute to 1-5x10^5 cells/ml with fresh medium. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.22x10^6 |
| Viability at cell freezing (%) | 91.2 | Antibiotics Used | free |
| Passage Number | P6 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | LD,PepB |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
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