JCRB0116.2 U937 cl1-22

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0116.2 Cell Name   U937 cl1-22
Profile   lymphoma, histiocytic, low susceptible clone of U937 for HIV Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   diffuse histiocytic lymphoma, clone of U937. Metastasis   
Tissue Metastasized    Genetics   express many monocyte-like characteristics
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   low susceptible clone for HIV virus
Classify   tumor Established by   Tabata,K.
Registered by   Ohnishi,K. Regulation for Distribution   free
Comment   Genetic ID was the same as the JCRB9021 U937 (EV=0.929). Year   1992
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   dilution
Cell Number on Passage   5 x 10^4 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   pleural effusion
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2811
Reference
Pubmed id:7381208Chemotactically responsive and nonresposive forms of a continuous human monocyte cell line.
Fischer DG,Pike MC,Koren HS,Snyderman R
J Immunol. 1980 Jul;125(1):463-5
Pubmed id:1083890Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines.
Ralph P,Moore MA,Nilsson K
J Exp Med. 1976 Jun 1;143(6):1528-33
Pubmed id:178611Establishment and characterization of a human histiocytic lymphoma cell line (U-937).
Sundström C,Nilsson K
Int J Cancer. 1976 May 15;17(5):565-77

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LOT Information

Cell No.   JCRB0116.2 Cell Name   U937 cl1-22
LOT No.   012194 Lot Specification   distribution
Medium   RPMI-1640 medium with 10% heat inactivated serum. Temperature   37 C
Cell Density at Seeding   1-5x10^5 cells/ml Methods for Passages   Simple dilution.
Doubling Time   26.1hr Cell Number in Vial (cells/1ml)   6.8x10^6
Viability at cell freezing (%)   94.1 Antibiotics Used   free
Passage Number   P8 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LD,PepB,G6PD, NP
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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