JCRB0122 KO51

Cell information

Important Notice(s)

Cross-contamination between KO51(JCRB0112) and K562

JCRB No.   JCRB0122 Cell Name   KO51
Profile   Stop providing for the issue of quality control. (Please contact us for more information) The cell line was reorted as an acute myeloblastic leukemia cell line but the cell bank detected the cell line deposited was genetically identical with the K562. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   46
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   acute myeloblastic leukeima/M2 Metastasis   No
Tissue Metastasized    Genetics   48,XY,+1,+2,7q-,21q- by G banding. CD13,CD33,CD41.
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   No N-ras mutation detected.
Classify   tumor Established by   Abo,J. & Inokuchi,K. et.al.
Registered by   Inokuchi,K. Regulation for Distribution   
Comment   Seed culture established. This cell line was found to be cross-contaminated with the K562 cell line. This will be eliminated from the DB and the revised cell line will be deosited from the original developper (Inokuchi, K.). Year   1997
Medium   Alpha-MEM with 10% heat-inactivated fetal calf serum. Methods for Passages   Simple dilution.
Cell Number on Passage   5x10^5 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   bone marrow
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3000
Pubmed id:7693050p53 and N-ras mutations in two new leukemia cell lines established from a patient with multilineage CD7-positive acute leukemia.
Abo J,Inokuchi K,Dan K,Nomura T
Blood. 1993 Nov 1;82(9):2829-36


LOT Information

Cell No.   JCRB0122 Cell Name   KO51
LOT No.   062597 Lot Specification   distribution
Medium   Alpha-MEM with 10% heat-inactivated fetal calf serum (Mitsubishi,PVF01). Temperature   37 C
Cell Density at Seeding   5x10^5 cells/ml Methods for Passages   Simple dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.3x10^6
Viability at cell freezing (%)   85.0 Antibiotics Used   free
Passage Number   P4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   TN Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Growth medium with 5% DMSO. CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information