Cell information

Important Notice(s)

Cross-contamination between KOSC-3 (JCRB0127) and Ca9-22

JCRB No.   JCRB0127 Cell Name   KOSC-3
Profile   Stop providing for the issue of quality control. (Please contact us for more information) STR-PCR analysis indicated that this cell line is the same cell line with the Ca9-22 (JCRB0625). Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   72
Identity   available Tissue for Primary Cancer   oral
Case history   oral cancer at stage III. Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Well differentiated squamous-cell carcinoma.
Classify   tumor Established by   Inagaki,T.
Registered by   Inagaki,T. Regulation for Distribution   free
Comment   Cells were established through xenografted tumors in nude mice by Geneticin treatment to eliminate mouse fibroblast cells. Year   1994
Medium   RPMI-1640 medium with 10% fetal bovine serum. Methods for Passages   Cells are passaged twice a week by trypsinization.
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   lower gingiva
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2789
Pubmed id:8314315Establishment of human oral-cancer cell lines (KOSC-2 and -3) carrying p53 and c-myc abnormalities by geneticin treatment.
Inagaki T,Matsuwari S,Takahashi R,Shimada K,Fujie K,Maeda S
Int J Cancer. 1994 Jan 15;56(2):301-8


LOT Information

Cell No.   JCRB0127 Cell Name   KOSC-3
LOT No.   062398 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (Rehatuin LB94203). Temperature   37 C
Cell Density at Seeding   1.7 x 10^4 cells/sq.cm. Methods for Passages   Cells are harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability at cell freezing (%)   95.2 Antibiotics Used   free
Passage Number   P53 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, LD, NP
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information