JCRB0128 TK-1

Cell information

Important Notice(s)

Cross-Culture contamination of TK-1 with U251 / JCRB0128 TK-1とU-251MGのクロスコンタミ

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0128 Cell Name   TK-1
Profile   Stop providing for the issue of quality control. (Please contact us for more information) Human glioblastoma multiforme cell line. Cross-Culture contamination with U251 suspected. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   22
Identity   available Tissue for Primary Cancer   neural
Case history   glioblastoma multiforme Metastasis   No
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   NT
Morphology   fibroblast-like Character   
Classify   tumor Established by   Tsuboi,K.
Registered by   Tsuboi,K. Regulation for Distribution   
Comment   STR-PCR experiments suggested the cross-culture contamination with the U251 (RCB0461). Year   1998
Medium    Methods for Passages   
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   brain
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_B325
Pubmed id:9183640[Establishment of human glioblastoma cell line "TK-1"].
Tsuboi K,Yoshii Y,Nose T
Hum Cell. 1996 Jun;9(2):125-8


LOT Information

Cell No.   JCRB0128 Cell Name   TK-1
LOT No.   063098 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (lot.Rehatuin LB94203). Temperature   37 C
Cell Density at Seeding   1.6 x 10^4 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA. Subculture every 4-5 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   89.0 Antibiotics Used   free
Passage Number   P10* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), LD, NP. Human.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information