JCRB0134 MCF-7

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0134 Cell Name   MCF-7
Profile   Human metastatic mammary carcinoma cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   Sapiens
Sex   F Age   
Identity   available Tissue for Primary Cancer   mammary
Case history   breast cancer Metastasis   Yes
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   dome formation observed
Classify   tumor Established by   Soule,H.D. et.al.
Registered by   Tanaka,N. Regulation for Distribution   
Comment    Year   1998
Medium   Eagle's minimum essetial medium with non-essential amino acids, 1 mM sodium pyruvate and 0.01 mg/ml insulin with 10% fetal calf serum. Methods for Passages   Cells are harvested after 0.25% trypsin and 0.02% EDTA treatment.
Cell Number on Passage   5 x 10^4 cells/sq.cm. Race   
CO2 Conc.   5 % Tissue Sampling   pleural effusion
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0031
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 905946
Reference
Pubmed id:14758095 Relevance of breast cancer cell lines as models for breast tumours: an update.
Lacroix M,Leclercq G
Breast Cancer Res Treat. 2004 Feb;83(3):249-89
Pubmed id:8118449 Retraction of cultured endothelial cell monolayer by human breast cancer cells, MCF-7.
Yoshioka K,Mukai M,Shinkai K,Akedo H
Cell Biol Int. 1993 Dec;17(12):1053-63
Pubmed id:1463869 In vitro potentiation by lonidamine of the cytotoxic effect of adriamycin on primary and established breast cancer cell lines.
Savini S,Zoli W,Nanni O,Volpi A,Frassineti GL,Magni E,Flamigni A,Amadori A,Amadori D
Breast Cancer Res Treat. 1992;24(1):27-34
Pubmed id:2137009 p53 gene mRNA expression and chromosome 17p allele loss in breast cancer.
Thompson AM,Steel CM,Chetty U,Hawkins RA,Miller WR,Carter DC,Forrest AP,Evans HJ
Br J Cancer. 1990 Jan;61(1):74-8
Pubmed id:2161818 Preparation of new 7-hydroxyguanine derivatives and their biological activities.
Kitahara M,Shinjyo K,Fukae M,Hosoe K,Shiraishi T,Ishii K,Watanabe K,Kawaharada H
J Antibiot (Tokyo). 1990 Apr;43(4):352-6
Pubmed id:2317801 Estradiol sensitivity test using contact-sensitive plates of confluent BALB/c 3T3 cell monolayers.
Matsuoka H,Ueo H,Yano K,Kido Y,Shirabe K,Mitsudomi T,Sugimachi K
Cancer Res. 1990 Apr 1;50(7):2113-8
Pubmed id:3224823 An estrogen-responsive element from the 5'-flanking region of the rat prolactin gene functions in MCF-7 but not in HeLa cells.
Somasekhar MB,Gorski J
Gene. 1988 Sep 15;69(1):23-8
Pubmed id:3038515 Localization of the mcf.2 transforming sequence to the X chromosome.
Noguchi T,Mattei MG,Oberlè I,Planche J,Imbert J,Pelassy C,Birg F,Birnbaum D
EMBO J. 1987 May;6(5):1301-7
Pubmed id:3458212 Phenol red in tissue culture media is a weak estrogen: implications concerning the study of estrogen-responsive cells in culture.
Berthois Y,Katzenellenbogen JA,Katzenellenbogen BS
Proc Natl Acad Sci U S A. 1986 Apr;83(8):2496-500
Pubmed id:6578363 Cytogenetic studies of human breast cancer lines: MCF-7 and derived variant sublines.
Whang-Peng J,Lee EC,Kao-Shan CS,Seibert K,Lippman M
J Natl Cancer Inst. 1983 Oct;71(4):687-95
Pubmed id:4357757 A human cell line from a pleural effusion derived from a breast carcinoma.
Soule HD,Vazguez J,Long A,Albert S,Brennan M
J Natl Cancer Inst. 1973 Nov;51(5):1409-16

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LOT Information

Cell No.   JCRB0134 Cell Name   MCF-7
LOT No.   12032010 Lot Specification   distribution
Medium   MEM(GIBCO) with non-essential amino acids,1 mM Na-pyruvate,10 ug/ml insulin and 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   5.0-9.0x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and PBS.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.87x10^6
Viability at cell freezing (%)   94.3 Antibiotics Used   free
Passage Number   p10* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:11
D7S820:8,9
D16S539:11,12
VWA:14,15
TH01:6
AM:X
TPOX:9,12
CSF1PO:10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0134 Cell Name   
LOT No.   05282009 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 0.1 mM non-essential amino acids, 1 mM sodium pyruvate, 10 ug/ml insulin and 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx 1.5 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   52 - 60 hrs Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   97.8 Antibiotics Used   free
Passage Number   Unknown (10 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0134 Cell Name   
LOT No.   07282008 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids (0.1 mM each), 1 mM sodium pyruvate, 10 ug/ml insulin, and 10% fetal bovine serum (GIBCO Cat. # 10099, lot 484375) Temperature   37 C
Cell Density at Seeding   2 - 6 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   6.6 x 10^6
Viability at cell freezing (%)   96.1 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0134 Cell Name   
LOT No.   03092007 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids (0.1 mM each), 1 mM sodium pyruvate, 10 ug/ml insulin (human recombinant) and 10% fetal bovine serum (FBS lot GIBCO 484375) Temperature   37 C
Cell Density at Seeding   1.5-3.0 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA (ラベルに細胞名がMKF-7と記載されている製品がございますが、MCF-7が正しい細胞名です。凍結後に判明したため貼り替えができませんでした。深くお詫び申し上げます。)
Doubling Time   50-60 hrs Cell Number in Vial (cells/1ml)   3.2 x 10^6
Viability at cell freezing (%)   92 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP and G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0134 Cell Name   
LOT No.   09112004 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids, sodium-pyruvate, 10 ug/ml insulin and 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   2 - 3 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   ca 2.5 days Cell Number in Vial (cells/1ml)   3.0 x 10^6
Viability at cell freezing (%)   90.0 Antibiotics Used   free
Passage Number   P10* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0134 Cell Name   
LOT No.   02122003 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids, 1 mM sodium pyruvate, 10 ug/ml insulin and 10% fetal bovine serum Temperature   37 C
Cell Density at Seeding   2 x 10^4 cells/sq.cm Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   D.T. = 48 hrs (max.) Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   85 Antibiotics Used   free
Passage Number   Unknown (9 at cellbank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   Culture medium with 20% FBS and 10% DMSO CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0134 Cell Name   
LOT No.   02272001 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum, non-essential amino acids, 1 mM sodium pyruvate and 10 ug/ml insulin Temperature   37 C
Cell Density at Seeding   5-10 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in PBS
Doubling Time   slow Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability at cell freezing (%)   83 Antibiotics Used   free
Passage Number   P12* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS in culture medium CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0134 Cell Name   MCF-7
LOT No.   041499 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 1.5g/L NaHCO3, NEAA, 1mM Pyrvate, 0.01mg/ml Insulin, and 10% FCS. Temperature   37 C
Cell Density at Seeding   5 x 10^4 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   DT=39.5 hrs Cell Number in Vial (cells/1ml)   2.1 x 10^6
Viability at cell freezing (%)   99.2 Antibiotics Used   free
Passage Number   P8* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, NP, LD. G6PD = type B. human.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5%
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0134 Cell Name   MCF-7
LOT No.   11192015 Lot Specification   distribution
Medium   Eagle's minimum essetial medium with non-essential amino acids, 1 mM sodium pyruvate, 10 ug/ml insulin and 10% fetal bovine serum (FBS; GIBCO Cat. # 10091). Temperature   37 C
Cell Density at Seeding   0.8 - 1.3 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 38 hrs. Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   89 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   89
Additional information   
Cell No.   JCRB0134 Cell Name   MCF-7
LOT No.   12192018 Lot Specification   distribution
Medium   Eagle's minimum essetial medium with non-essential amino acids, 1 mM sodium pyruvate, 10 ug/ml insulin and 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   1.0 - 1.9 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   95.4 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   85.2
Additional information   
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