JCRB0135 MT-4

Cell information

Cell type:virus production cells (View Pricing Information)

JCRB No.   JCRB0135 Cell Name   MT-4
Profile   HTLV-I-transformed leukocytes, HTLV-I antigen-positive. (This cell line is supplied from National Institute of Biomedical Innovation.) Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   
Classify   tumor Established by   
Registered by    Regulation for Distribution   
Comment    Year   1992
Medium    Methods for Passages   
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
HHV6 - HTLV-1 +
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2632
Pubmed id:11336546Induction of Tax i expression in MT-4 cells by 5-azacytidine leads to protein binding in the HTLV-1 LTR in vivo.
Datta S,Kothari NH,Fan H
Virology. 2001 May 10;283(2):207-14
Pubmed id:8927062[Cloning proviral DNA sequences of the human T-cell leukemia virus (HTLV-1) from the genome of cultured MT-4 cells].
Susloparov MA,Krendel'shchikov AV,Bakhtina MM,Bedristov AI,Babkin IV,Gutorov VV,Eroshkin AM,Mamaeva OA
Mol Gen Mikrobiol Virusol. 1996 Apr-Jun;(2):32-9
Pubmed id:7988730Autoantigen Ku protein is involved in DNA binding proteins which recognize the U5 repressive element of human T-cell leukemia virus type I long terminal repeat.
Okumura K,Takagi S,Sakaguchi G,Naito K,Minoura-Tada N,Kobayashi H,Mimori T,Hinuma Y,Igarashi H
FEBS Lett. 1994 Dec 12;356(1):94-100


LOT Information

Cell No.   JCRB0135 Cell Name   
LOT No.   102799 Lot Specification   distribution
Medium   RPMI1640 medium with 10% triptose phosphate broth and 10% fetal bovine serum (BioWhittaker 6M0122) Temperature   37 C
Cell Density at Seeding   1.3 x 10^5 cells/ml Methods for Passages   dilution
Doubling Time   D.T. = ca 30 hrs Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   Unknown (5 at cellbank) PDL   
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   Culture medium contaning 10% DMSO CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information