JCRB0149 Bhas 42

Cell information

Important Notice(s)

Balb/c 3T3 A31-1に派生する細胞株の由来の問題について

The problem of origin of Balb/c 3T3 A31-1 derived cell lines.

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0149 Cell Name   Bhas 42
Profile   BALB 3T3 A31-1-1 cell line transfected with H-ras gene. The cell line keeps characteristics of the contact inhibition and the transfomation is induced by phorbol ester alone. No initialization required. (Strain problem was found. Refer Important Notice at cell detail page.) Other Name   
Animal   mouse Strain   Swiss
Genus   Mus Species   musclus
Sex    Age   embryo
Identity    Tissue for Primary Cancer   embryo
Case history   normal Metastasis   
Tissue Metastasized    Genetics   H-ras gene transfected BALB/c 3T3 A31-1-1 cell line. The origin of BALB/c 3T3 A31-1 was found not to be derived from BALB 3T3 A31.
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   Growth on the dish is inhibited by cell to cell contact. Phorbol ester alone is enough to induce cellular transformation.
Classify   transfected Established by   Sasaki,K.
Registered by   Sasaki,K. Regulation for Distribution   free
Comment   Appearing of naturally occured transformation depends on serums and passage methods. Year   2000
Medium   Eagle's minimal essential medium with 10% fetal bovine serum. Methods for Passages   Cells are harvested after treatment with 0.25% trypsin and 0.02% EDTA. Split ratio is practically 1/100. Harvest cells before confluent at subculture.
Cell Number on Passage   2 x 10^4 cells/100mm dish. Race   
CO2 Conc.   5 % Tissue Sampling   whole body of embryo
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_5494
Pubmed id:14729374An assay method for the prediction of tumor promoting potential of chemicals by the use of Bhas 42 cells.
Ohmori K,Sasaki K,Asada S,Tanaka N,Umeda M
Mutat Res. 2004 Feb 14;557(2):191-202
Pubmed id:8481899Tumor necrosis factor acts as a tumor promoter in BALB/3T3 cell transformation.
Komori A,Yatsunami J,Suganuma M,Okabe S,Abe S,Sakai A,Sasaki K,Fujiki H
Cancer Res. 1993 May 1;53(9):1982-5
Pubmed id:20702251Transformation of ras transfected BALB 3T3 clone (Bhas 42) by promoters: Application for screening and specificity of promoters.
Sasaki K,Mizusawa H,Ishidate M,Tanaka N
Toxicol In Vitro. 1990;4(4-5):657-9
Pubmed id:3141328Isolation and characterization of ras-transfected BALB/3T3 clone showing morphological transformation by 12-O-tetradecanoyl-phorbol-13-acetate.
Sasaki K,Mizusawa H,Ishidate M
Jpn J Cancer Res. 1988 Aug;79(8):921-30

LOT Information

Cell No.   JCRB0149 Cell Name   Bhas 42
LOT No.   11142013 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (FBS; GIBCO Cat # 10099). Temperature   37C
Cell Density at Seeding   1.2 - 3.2 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Split ratio is practically 1/100. Harvest cells before confluent at subculture.
Doubling Time    Cell Number in Vial (cells/1ml)   2.3 x 10^6 (cells/1 mL)
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   P15 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,NP,LDH were examined. Mouse confirmed.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   5%
Viability immediately after thawing (%)   96.7 Additional information