JCRB0155 NM-1

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0155 Cell Name   NM-1
Profile   Newly established primary bovine cell line established from artery which is confirmed to be free from BVDV contamination. Other Name   
Animal   bovine Strain   
Genus   Bos Species   taurus
Sex    Age   fetus
Identity   not done Tissue for Primary Cancer   vascular system
Case history   normal Metastasis   
Tissue Metastasized    Genetics   Normal epitherum
Life Span   finite Crisis PDL   PDL=110
Morphology   epithelial-like Character   Pestivirus free primary bovine cells.
Classify   primary Established by   Nagisa,S. & Abe,T.
Registered by   Abe,T. Regulation for Distribution   free
Comment   Cells established by MHW research fund. Smooth muscle actin(SMA) observed in 15% of total cells. Year   2000
Medium   Eagle's minimal essential medium with 10% fetal calf serum (Mitsubishi PFCS PVF001). It is important to use the BVDV free serum to keep this cell line free from BVDV. Methods for Passages   Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Cell Number on Passage   1.5 x 10^2 cells/sq.cm Race   
CO2 Conc.   5 % Tissue Sampling   arterial endothelium
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3074
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LOT Information

Cell No.   JCRB0155 Cell Name   
LOT No.   02142008 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat.# 10099 lot 484375) Temperature   37 C
Cell Density at Seeding   1.3 - 1.7 x 10^4 cells/sq.cm Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   30 hrs Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   86 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as bovine by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0155 Cell Name   NM-1
LOT No.   12112000 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal calf serum(Mitsubishi PFCS PVF001). BVDV free serum recommended. Temperature   37 C
Cell Density at Seeding   1.0 x 10^4 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA. Change medium every 3-4 days and subculture once a week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.4 x 10^6
Viability at cell freezing (%)   92.9 Antibiotics Used   free
Passage Number   P6* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, NP and LDH examined. Bovine.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium containing 5% DMSO. CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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