JCRB0166 HMC-1-8

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0166 Cell Name   HMC-1-8
Profile   A target cell of the T-cell killer clone T(cHMC-1). Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   35
Identity   available Tissue for Primary Cancer   mammary
Case history   a person had undergone left radical mastectomy 3 years previously for an infiltrating scirrhous carcinoma of the left breast. Metastasis   Yes
Tissue Metastasized   pleural effusion Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   HMC-1-8 is a target cell of the T-cell killer clone T(cHMC-1) derived from breast cancer established from pleural effusion.
Classify   tumor Established by   Kikuchi,H.
Registered by   Sato,T. Regulation for Distribution   not described
Comment    Year   2001
Medium   RPMI1640 medium with 10% fetal bovine serum. Methods for Passages   Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA at room temperature for 10 minutes. Subculture once a week and change medium twice a week.
Cell Number on Passage   8 x 10^4 cells/ml Race   Japanese
CO2 Conc.   5 % Tissue Sampling   pleural effusion
Virus Summary   tested Virus Tested   CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-) [-/negative, +/positive, nt/not tested]
Tissue Type   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2949
Reference
Pubmed id:2786747 Identification by monoclonal antibody of the tumor antigen of a human autologous breast cancer cell that is involved in cytotoxicity by a cytotoxic T-cell clone.
Okubo M,Sato N,Wada Y,Takahashi S,Torimoto K,Takahashi N,Sato T,Okazaki M,Asaishi K,Kikuchi K
Cancer Res. 1989 Jul 15;49(14):3950-4
Pubmed id:3488124 Specific cytotoxicity of a long-term cultured T-cell clone on human autologous mammary cancer cells.
Sato T,Sato N,Takahashi S,Koshiba H,Kikuchi K
Cancer Res. 1986 Sep;46(9):4384-9

Images



LOT Information

Cell No.   JCRB0166 Cell Name   HMC-1-8
LOT No.   05222001 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (Intergen RB52405). Temperature   37 C
Cell Density at Seeding   8.0 x 10^4 cells/ml Methods for Passages   Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA at room temp. for 10 min. Subculture once a week and medium change twice a week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.0 x 10^6
Viability at cell freezing (%)   96.7 Antibiotics Used   free
Passage Number   P17* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0166 Cell Name   HMC-1-8
LOT No.   12052013 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10091). Temperature   37C
Cell Density at Seeding   0.9 - 1.7 x 10^5 cells/mL Methods for Passages   Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA at room temperature for 10 minutes. Subculture once a week and change medium twice a week.
Doubling Time    Cell Number in Vial (cells/1ml)   4.2 x 10^6 (cells/1
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   Unknown (20 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB),NP,LDH were examined. Human,not HeLa
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   93.3
Additional information   
CLOSE