JCRB0177.1 KP-1NL

Cell information

Important Notice(s)

JCRB0177.0 KP-1N, JCRB0177.1 KP-1NL細胞の由来の問題について

Problem of identity in JCRB0177.0 KP-1N and JCRB0177.1 KP-1NL.

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0177.1 Cell Name   KP-1NL
Profile   Stop providing for the issue of quality control. (Please contact us for more information) Cells established from the resulting tumor nodules in the liver of mouse, into the spleen where KP-1N cells had been injected. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   69
Identity   available Tissue for Primary Cancer   pancreas
Case history   pancreatic cancer Metastasis   Yes
Tissue Metastasized   liver Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Cells were established from the resulting tumor nodules in the liver of mouse, into the spleen where KP-1N cells had been injected.
Classify   tumor Established by   Kono,A. et.al.
Registered by   Iguchi,H. Regulation for Distribution   
Comment   Cells were identified to be unique on 10/17/2001 by STR-PCR. Year   2001
Medium   RPMI1640 medium with 10% fetal bovine serum. Methods for Passages   Cells are harvested after treatment with 0.02% EDTA and 0.1% trypsin. Subculture when cells reach to subconfluent.
Cell Number on Passage   1-2 x 10^5 cells/T25 frask Race   Japanese
CO2 Conc.   5 % Tissue Sampling   pancreas
Virus Summary   teste Virus Tested   GAPDH(+), CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), HBV(-), parvoB19(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-)[-/negative, +/positive, nt/not tested, GAPDH = positive control]
Tissue Type   
Reference
Pubmed id:9735394 Alteration of the CDKN2A gene in pancreatic cancers: Is it a late event in the progression of pancreatic cancer?
Sugimoto Y,Morita R,Hikiji K,Imura G,Ogata Y,Yasuda D,Kono A,Iguchi H
Int J Oncol. 1998 Oct;13(4):669-76
Pubmed id:21607521 Alterations of the p53 tumor-suppressor gene and ki-ras oncogene in human pancreatic cancer-derived cell-lines with different metastatic potential.
Iguchi H,Morita R,Yasuda D,Takayanagi R,Ikeda Y,Takada Y,Shimazoe T,Nawata H,Kono A
Oncol Rep. 1994 Nov;1(6):1223-7
Pubmed id:2172194 Establishment and characterization of human pancreatic cancer cell lines in tissue culture and in nude mice.
Ikeda Y,Ezaki M,Hayashi I,Yasuda D,Nakayama K,Kono A
Jpn J Cancer Res. 1990 Oct;81(10):987-93

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LOT Information

Cell No.   JCRB0177.1 Cell Name   
LOT No.   05282004 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   1 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   30 - 40 hrs Cell Number in Vial (cells/1ml)   2.4 x 10^6
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   P11* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0177.1 Cell Name   KP-1NL
LOT No.   10202001 Lot Specification   distribution
Medium   RPMI1640 medium with 10 % fetal calf serum (Intergen RB52405). Temperature   37 C
Cell Density at Seeding   2.0 x 10^4 cells/sq.cm. Methods for Passages   Cells were harvested after treatment with 0.1% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   98.7 Antibiotics Used   free
Passage Number   P7* PDL   NT
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   G6PD(typeB), NP, LDH examined. Human not HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium containing 5% DMSO. CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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