-Detailed Information [JCRB0182]-

JCRB0182 KP-4

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	JCRB0182	Cell Name	KP-4
Profile	Human tumor cell line secreting parathyroid hormone-related peptide (PTHrP) established from pancreatic tumor.	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	M	Age	50
Identity	available	Tissue for Primary Cancer	pancreas
Case history	pancreatic ductal cell carcinoma	Metastasis	Yes
Tissue Metastasized	ascites	Genetics
Life Span	infinite	Crisis PDL
Morphology	epithelial-like	Character	Tumor cell line secreting parathyroid hormone-related peptide (PTHrP). Transplantable to nude mice.
Classify	tumor	Established by	Nishi,Y. et.al.
Registered by	Iguchi,H. et.al.	Regulation for Distribution	non
Comment	Cells are observed to be unique from the STR-PCR analysis.	Year	2001
Medium	Iscove's modified Dulbecco's medium with 20% fetal calf serum (original description). Current medium for propagation is RPMI 1640 medium with 10% fetal calf serum.	Methods for Passages	Cells were prepared after treatment with 0.25% trypsin and 0.1% EDTA.
Cell Number on Passage		Race	Japanese
CO2 Conc.		Tissue Sampling	pancreas
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_1338
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link.	753572

Reference
Pubmed id:9613452	Effect of retinoic acid on morphological changes of human pancreatic cancer cells on collagen gels: a possible association with the metastatic potentials. Jimi S,Shono T,Tanaka M,Kono A,Yamada Y,Shudo K,Kuwano M Oncol Res. 1998;10(1):7-14
Pubmed id:21559554	Establishment and characterization of pthrp-producing human pancreatic-cancer cell-line. Nishi Y,Haji M,Takayanagi R,Iguchi H,Shimazoe T,Hirata J,Nawata H Int J Oncol. 1994 Jul;5(1):33-9

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LOT Information

12052011
01262006
01162018
10262020

Cell No.	JCRB0182	Cell Name
LOT No.	12052011	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum (FBS, GIBCO Cat. # 10099)	Temperature	37 C
Cell Density at Seeding	7 - 10 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	13 hrs ? (<24 hrs.)	Cell Number in Vial (cells/1ml)	3.3 x 10^6
Viability at cell freezing (%)	94.8	Antibiotics Used	free
Passage Number	Unknown (8 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI 1640	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0182	Cell Name
LOT No.	01262006	Lot Specification	distribution
Medium	RPMI1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 4218333S)	Temperature	37 C
Cell Density at Seeding	0.5 - 1.7 x 10^5 cells/ml	Methods for Passages	0.25% trypsin and 0.1% EDTA
Doubling Time	approx. 31 hrs	Cell Number in Vial (cells/1ml)	1.6 x 10^6
Viability at cell freezing (%)	98.9	Antibiotics Used	free
Passage Number	Unknown (P8 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI1640	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0182	Cell Name	KP-4
LOT No.	01162018	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal Bovine Serum (FBS; Sigma Cat. # 172012).	Temperature	37 C
Cell Density at Seeding	5.5 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 22 hrs.	Cell Number in Vial (cells/1ml)	3.3 x 10^6
Viability at cell freezing (%)	94	Antibiotics Used	free
Passage Number	Unknown (10 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI 1640	CO2 Conc.	5%
Viability immediately after thawing (%)	94	Additional information

Cell No.	JCRB0182	Cell Name	KP-4
LOT No.	10262020	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012)	Temperature	37 C
Cell Density at Seeding	approx. 5 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 44 hrs.	Cell Number in Vial (cells/1ml)	1.1 x 10^6
Viability at cell freezing (%)		Antibiotics Used	free
Passage Number	Unknown (7 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI 1640	CO2 Conc.	5%
Viability immediately after thawing (%)	97.4	Additional information