JCRB0211 A9
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0211 | Cell Name | A9 |
|---|---|---|---|
| Profile | skin fibroblast, 8-azaguanine-resistant, (C3H mouse) | Other Name | A9 |
| Animal | mouse | Strain | C3H |
| Genus | Mus | Species | musclus |
| Sex | Age | ||
| Identity | not done | Tissue for Primary Cancer | skin |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | HPRT and APRT deficient. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | Character | skin fibroblast, AG-r | |
| Classify | Established by | Littlefield,J.W. | |
| Registered by | Isaka,H. | Regulation for Distribution | |
| Comment | Resistant to 8-azaguanine. | Year | 1985 |
| Medium | Eagle's minimal essential medium with 10% fetal calf serum | Methods for Passages | Cells are treated with 0.25% trypsin. |
| Cell Number on Passage | 2 x 10^4 cells/ml | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | skin |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:14213660 | THREE DEGREES OF GUANYLIC ACID--INOSINIC ACID PYROPHOSPHORYLASE DEFICIENCY IN MOUSE FIBROBLASTS. LITTLEFIELD JW Nature. 1964 Sep 12;203():1142-4 |
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LOT Information
| Cell No. | JCRB0211 | Cell Name | A9 |
|---|---|---|---|
| LOT No. | 07122006 | Lot Specification | distribution |
| Medium | Eagle's minimal essential medium with 10% fetal bovine serum (FBS GIBCO-catalog#10099, lot4218333S) | Temperature | 37 C |
| Cell Density at Seeding | 2 - 10 x 10^4 cells/ml | Methods for Passages | 0.25% trypsin |
| Doubling Time | approx. 1 day | Cell Number in Vial (cells/1ml) | 4.1 x 10^6 |
| Viability at cell freezing (%) | 98.2 | Antibiotics Used | free |
| Passage Number | P7 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as mouse by NP, G6PD, AST. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - EMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0211 | Cell Name | |
|---|---|---|---|
| LOT No. | 031792 | Lot Specification | distribution |
| Medium | MEM : 10%FCS | Temperature | 37 |
| Cell Density at Seeding | Methods for Passages | ||
| Doubling Time | Cell Number in Vial (cells/1ml) | ||
| Viability at cell freezing (%) | Antibiotics Used | ||
| Passage Number | PDL | ||
| Sterility: MYCOPLASMA | Sterility: BACTERIA | ||
| Sterility: FUNGI | Isozyme Analysis | ||
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |