JCRB0225 COLO320 DM

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0225 Cell Name   COLO320 DM
Profile   Human colon carcinoma cell line with double minute chromosomes. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   55
Identity   available Tissue for Primary Cancer   colon
Case history   carcinoma of the sigmoid colon Metastasis   
Tissue Metastasized    Genetics   colon, adenocarcinoma
Life Span   infinite Crisis PDL   
Morphology   round shaped Character   colon, adenocarcinoma
Classify   tumor Established by   Quinn,L.A.
Registered by   Egashira, Y. Regulation for Distribution   
Comment    Year   1987
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum. Methods for Passages   Cells are treated with 0.25% trypsin.
Cell Number on Passage   2x10^4 cells/ml Race   Caucasian
CO2 Conc.   5 % Tissue Sampling   colon
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0219
Reference
Pubmed id:14720324 Expression of VEGF-C and VEGF-D at the invasive edge correlates with lymph node metastasis and prognosis of patients with colorectal carcinoma.
Onogawa S,Kitadai Y,Tanaka S,Kuwai T,Kimura S,Chayama K
Cancer Sci. 2004 Jan;95(1):32-9
Pubmed id:2085852 Flavonoids inhibit the expression of heat shock proteins.
Hosokawa N,Hirayoshi K,Nakai A,Hosokawa Y,Marui N,Yoshida M,Sakai T,Nishino H,Aoike A,Kawai K
Cell Struct Funct. 1990 Dec;15(6):393-401
Pubmed id:2479441 Multiple mechanisms in the motor responses of the guinea-pig isolated urinary bladder to bradykinin.
Maggi CA,Patacchini R,Santicioli P,Geppetti P,Cecconi R,Giuliani S,Meli A
Br J Pharmacol. 1989 Oct;98(2):619-29
Pubmed id:498117 Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions.
Quinn LA,Moore GE,Morgan RT,Woods LK
Cancer Res. 1979 Dec;39(12):4914-24

Images



LOT Information

Cell No.   JCRB0225 Cell Name   
LOT No.   09142004 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 X
Cell Density at Seeding   1.4 - 2.8 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Some cells grow in suspension by forming small cell aggregates. At passage, collect the floating cells as well as adherent cells.
Doubling Time   23 hrs Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   96 Antibiotics Used   free
Passage Number   P10 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0225 Cell Name   COLO320 DM
LOT No.   112996 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (ICN40224155). Temperature   37 C
Cell Density at Seeding   1-2x10^5 cells/ml Methods for Passages   Wash cells with PBS(-) and treat them with 0.02% EDTA and 0.25% trypsin for 5 min at 37 C.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.4x10^6
Viability at cell freezing (%)   96.0 Antibiotics Used   free
Passage Number   P10 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12
D13S317:11
D7S820:9,12
D16S539:11,12
VWA:15,18
TH01:9
AM:X
TPOX:8,9
CSF1PO:11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0225 Cell Name   COLO320 DM
LOT No.   03242014 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   1.7 -3.5 x 10^4 cells/mL Methods for Passages   0.25% trypsin and 0.02% EDTA. Some cells grow in suspension by forming small cell aggregates. At passage, collect the floating cells as well as adherent cells.
Doubling Time    Cell Number in Vial (cells/1ml)   4.8 x 10^6
Viability at cell freezing (%)   93.8 Antibiotics Used   free
Passage Number   P11 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G5PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12
D13S317:11
D7S820:9,12
D16S539:11,12
VWA:15,18
TH01:9
AM:X
TPOX:8,9
CSF1PO:11
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   93.8
Additional information   
Cell No.   JCRB0225 Cell Name   
LOT No.   091994 Lot Specification   distribution
Medium   RPMI1640 + 10 % FCS Temperature   37
Cell Density at Seeding   1/5-1/10 Methods for Passages   0.25% trypsin and 0.02% EDTA Collecting of floating cells and attached cells
Doubling Time    Cell Number in Vial (cells/1ml)   1.1 x 10^7
Viability at cell freezing (%)   95.0 Antibiotics Used   free
Passage Number   P10 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0225 Cell Name   
LOT No.   092094 Lot Specification   distribution
Medium   RPMI1640 + 10 % FCS Collecting of floating cells and attached cells Temperature   37
Cell Density at Seeding   1/5-1/10 Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   1.1 x 10^7
Viability at cell freezing (%)   95.0 Antibiotics Used   free
Passage Number   P10 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
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