JCRB0226 COLO201

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0226 Cell Name   COLO201
Profile   adenocarcinoma Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   colon
Case history    Metastasis   
Tissue Metastasized    Genetics   colon, adenocarcinoma
Life Span    Crisis PDL   
Morphology   round Character   colon, adenocarcinoma
Classify    Established by   Semple,T.U. et.al.
Registered by   Egashira, Y. Regulation for Distribution   
Comment    Year   1987
Medium   RPMI1640 medium with 10% fetal calf serum. Methods for Passages   Cells are treated with 0.25% trypsin.
Cell Number on Passage   2x10^4 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   colon
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1987
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 905961
Reference
Pubmed id:14720324Expression of VEGF-C and VEGF-D at the invasive edge correlates with lymph node metastasis and prognosis of patients with colorectal carcinoma.
Onogawa S,Kitadai Y,Tanaka S,Kuwai T,Kimura S,Chayama K
Cancer Sci. 2004 Jan;95(1):32-9
Pubmed id:1980764Expression of several growth factors and their receptor genes in human colon carcinomas.
Ito M,Yoshida K,Kyo E,Ayhan A,Nakayama H,Yasui W,Ito H,Tahara E
Virchows Arch B Cell Pathol Incl Mol Pathol. 1990;59(3):173-8
Pubmed id:565251Tumor and lymphoid cell lines from a patient with carcinoma of the colon for a cytotoxicity model.
Semple TU,Quinn LA,Woods LK,Moore GE
Cancer Res. 1978 May;38(5):1345-55

Images



LOT Information

Cell No.   JCRB0226 Cell Name   
LOT No.   11252003 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   1 - 2 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Most of cells do not attach to substratum. Collect the non-adherent cells as wells as adherent cells at subcultivation.
Doubling Time   ca. 4 days Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   87.5 Antibiotics Used   free
Passage Number   P14 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0226 Cell Name   COLO201
LOT No.   090398 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (lot#.Summit 8.1E+41). Temperature   37 C
Cell Density at Seeding   2.0x10^4 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.8 x 10^6
Viability at cell freezing (%)   80.2 Antibiotics Used   free
Passage Number   P13 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP examined. Human confirmed.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0226 Cell Name   
LOT No.   070695 Lot Specification   distribution
Medium   RPMI1640 + 10 % FCS Temperature   37
Cell Density at Seeding   1/5 - 1/10 Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   97.2 Antibiotics Used   free
Passage Number   P12 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST, G6PD, LD, MPI, NP, PEPB
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0226 Cell Name   COLO201
LOT No.   12082014 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   3 x 10^5 cells/mL Methods for Passages   0.25% trypsin and 0.02% EDTA. Most of cells do not attach to substratum. Collect the non-adherent cells as wells as adherent cells at subcultivation.
Doubling Time   approx. 4 days Cell Number in Vial (cells/1ml)   8.0 x 10^5 cells
Viability at cell freezing (%)   84.1 Antibiotics Used   free
Passage Number   P15 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:10,13
D13S317:10,12
D7S820:9,10
D16S539:12,13
VWA:15
TH01:8,9
AM:X
TPOX:11
CSF1PO:11,12
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   84.1
Additional information   
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