JCRB0235 Lu-134-A-H

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0235 Cell Name   Lu-134-A-H
Profile   small cell carcinoma, classic type Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   80
Identity   available Tissue for Primary Cancer   lung (cancer)
Case history   small cell carcinoma Metastasis   
Tissue Metastasized    Genetics   small cell carcinoma
Life Span   infinite Crisis PDL   
Morphology    Character   small cell carcinoma, classic type
Classify   tumor Established by   Terasaki,T. & Shimosato,Y.
Registered by   Egashira, Y. Regulation for Distribution   
Comment   Cell number of the suculture is estimated roughly. Cell clumps cannot be eliminated. Year   1987
Medium   RPMI1640 medium with 10% fetal calf serum. Methods for Passages   dilution of cell clumps
Cell Number on Passage   1.5 x 10^6 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   lung
Virus Summary   tested Virus Tested   CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-) [-/negative, +/positive, nt/not tested]
Tissue Type   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1387
Reference
Pubmed id:3022030 Changes in cell characteristics due to culture conditions in cell lines from human small cell lung cancer.
Terasaki T,Shimosato Y,Nakajima T,Tsumuraya M,Morinaga S,Hirohashi S,Yamaguchi K,Kato K,Ichinose H,Nagatsu T
Jpn J Clin Oncol. 1986 Sep;16(3):203-12

Images



LOT Information

Cell No.   JCRB0235 Cell Name   Lu-134-A-H
LOT No.   080495 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (JRH.12103-78P) Temperature   37 C
Cell Density at Seeding   split to 1/3 Methods for Passages   suspend by pipetting
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.1x10^6
Viability (%)   93.6 Antibiotics Used   free
Passage Number   P7 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Additional information   
Images
Cell No.   JCRB0235 Cell Name   Lu-134-A-H
LOT No.   06112015 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091). Temperature   37 C
Cell Density at Seeding   2.5 - 10 x 10^5 cells/ml Methods for Passages   This cell line grow with forming cell aggregates (suspension culture). At passage, disparse the cell aggregate to small cell clumps by pipetting, and dilute to fresh medium.
Doubling Time   approx. 73 hrs Cell Number in Vial (cells/1ml)   3.7 x 10^6
Viability (%)   52.2 Antibiotics Used   free
Passage Number   Unknown (10 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11
D13S317:9
D7S820:11
D16S539:9
VWA:17
TH01:9
AM:X
TPOX:11
CSF1PO:12
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Additional information   
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