JCRB0254 MKN45

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0254 Cell Name   MKN45
Profile   adenocarcinoma, poorly differentiated Other Name   MKN45
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    F Age   62-year-old
Identity   available Tissue for Primary Cancer   stomach
Case history    Metastasis   Y
Tissue Metastasized   liver Genetics   stomach, 62 year-old female.
Life Span    Crisis PDL   
Morphology    Character   stomach, 62 year-old female.
Classify    Established by   Hojo,H.
Registered by   Motoyama & Watanabe Regulation for Distribution   
Comment   Poorly differentiated adenocarcinoma. Mycoplasma- eliminated cells by MC-210. Year   1989
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   Cells are treated with 0.02 % EDTA and 0.25% trypsin.
Cell Number on Passage   split ratio=1/10 Race   
CO2 Conc.   5 % Tissue Sampling   liver metastasis
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0434
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 925340
Reference
Pubmed id:2454734Selection of a monoclonal antibody reactive with a high-molecular-weight glycoprotein circulating in the body fluid of gastrointestinal cancer patients.
Sakurai Y,Hirohashi S,Shimosato Y,Kodaira S,Abe O
Cancer Res. 1988 Jul 15;48(14):4053-8
Pubmed id:3095279Detection of amplified DNA sequences in gastric cancers by a DNA renaturation method in gel.
Nakatani H,Tahara E,Yoshida T,Sakamoto H,Suzuki T,Watanabe H,Sekiguchi M,Kaneko Y,Sakurai M,Terada M
Jpn J Cancer Res. 1986 Sep;77(9):849-53
Pubmed id:noneEstablishment of cultured cell lines of human stomach cancer origin and their morphological characteristics.
Hojo H.
Niigata Igakkai Zasshi, 1977, 91:737-763

Images



LOT Information

Cell No.   JCRB0254 Cell Name   
LOT No.   05242012 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS, GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/mL Methods for Passages   This cell line is round morphology and loosely attaches to substratum. Harvest cells by pipetting or 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 24 hrs. Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability at cell freezing (%)   97.0 Antibiotics Used   free
Passage Number   P21 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   Yes
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0254 Cell Name   
LOT No.   05222009 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (used FBS lot, GIBCO Cat. # 10099, lot 484375) Temperature   37 C
Cell Density at Seeding   approx. 2 x 10^5 cells/ml Methods for Passages   This cell line is round morphology and loosely attaches to substratum. Harvest cells by pipetting or 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 26 hrs. Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   ~100 Antibiotics Used   free
Passage Number   P21 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0254 Cell Name   
LOT No.   10162007 Lot Specification   distribution
Medium   RPMI1640 +10% FBS(lot:GIBCO484375) Temperature   37C
Cell Density at Seeding   9.0 x 10^4cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in PBS
Doubling Time   20hr Cell Number in Vial (cells/1ml)   8.2 x 10^6 cells
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   P22 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human by G6PD(type B) NP, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   70% Culture medium + 20% FCS + 10% DMSO CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0254 Cell Name   
LOT No.   12162005 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   0.6 - 2 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. This cell line loosely attaches to substratum.
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   5.6 x 10^6
Viability at cell freezing (%)   98.1 Antibiotics Used   free
Passage Number   P21 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0254 Cell Name   
LOT No.   02172003 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS lot; BioWhittaker 0S069F) Temperature   37 C
Cell Density at Seeding   2 x 10^5 cells/ml. At thawing plate to T-75 flask. Methods for Passages   pipetting or 0.25% trypsin and 0.02% EDTA. This cell line loosely attaches to substratum.
Doubling Time   26 hrs Cell Number in Vial (cells/1ml)   4.6 x 10^6
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   P24 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS in RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0254 Cell Name   MKN45
LOT No.   022896 Lot Specification   distribution
Medium   RPMI1640 medium with 10% heat inactivated fetal bovine serum (JRS.CO19407). Temperature   37 C
Cell Density at Seeding   1x10^5/ml Methods for Passages   Cells washed once with PBS(-) and cells were treated with 0.25% trypsin & 0.02% EDTA for 5min at 37C
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.8x10^6
Viability at cell freezing (%)   89.0 Antibiotics Used   free
Passage Number   P21 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0254 Cell Name   MKN45
LOT No.   022189 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal calf serum. Temperature   37 C
Cell Density at Seeding   Split ratio = 1/10 Methods for Passages   Cells harvested after treatment with 0.02% EDTA and 0.25% trypsin and disperse completely.
Doubling Time   NT Cell Number in Vial (cells/1ml)   6x10^6
Viability at cell freezing (%)   96% Antibiotics Used   free
Passage Number   P14* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LD,G6PD,NP,PepB,AST,MD
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0254 Cell Name   
LOT No.   012198 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   1-3 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Cell attachment was poor and tended to form cell aggeregates.
Doubling Time   about 28 hrs Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   96.0 Antibiotics Used   free
Passage Number   P20 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0254 Cell Name   
LOT No.   091694 Lot Specification   distribution
Medium   RPMI 1640 + FCS 10% Temperature   37
Cell Density at Seeding   1/10 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time    Cell Number in Vial (cells/1ml)   2.4 x 10^6
Viability at cell freezing (%)   98.0 Antibiotics Used   free, Mycoplasma-eliminated previously by MC-210
Passage Number   P19 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST, G6PD, LD, MPI, NP, PEPB
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0254 Cell Name   MKN45
LOT No.   11172016 Lot Specification   distribution
Medium   RPMI1640 medium with 10% heat-inactivated fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.5 x 10^5 cells/mL Methods for Passages   This cell line is round morphology and loosely attaches to substratum. Both adherent and non-adherent cells should be recovered. Adherent cells are harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 26 hrs. Cell Number in Vial (cells/1ml)   3.2 x 10^6
Viability at cell freezing (%)   97 Antibiotics Used   free
Passage Number   P22 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   97
Additional information   
Cell No.   JCRB0254 Cell Name   MKN45
LOT No.   05202020 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.0 x 10^5 cells/mL Methods for Passages   This cell line is consisted of loosely attached cells and suspension cells. Both adherent and non-adherent cells should be recovered. Adherent cells are harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 27 hrs. Cell Number in Vial (cells/1ml)   3.2 x 10^6
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   P22 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Y/N Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   99
Additional information   
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