JCRB0255 MKN74

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0255 Cell Name   MKN74
Profile   Human cell line derived from stomach cancer. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   37
Identity   available Tissue for Primary Cancer   stomach
Case history   stomach cancer metastasized to liver Metastasis   Yes
Tissue Metastasized   liver Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   
Classify   tumor Established by   Hojo,H.
Registered by   Motoyama & Watanabe Regulation for Distribution   
Comment   Moderately differentiated tubular adenocarcinoma. Mycoplasma eliminated cells by MC-210. Year   1989
Medium   RPMI1640 medium with 10% heat inactivated fetal bovine serum. Methods for Passages   Cells are washed once with PBS(-) and treated with 0.02% EDTA and 0.25% trypsin for 10-15 min at 37 C.
Cell Number on Passage   split ratio=1/10 Race   Japanese
CO2 Conc.   5 % Tissue Sampling   liver metastasis
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2791
Reference
Pubmed id:2454734 Selection of a monoclonal antibody reactive with a high-molecular-weight glycoprotein circulating in the body fluid of gastrointestinal cancer patients.
Sakurai Y,Hirohashi S,Shimosato Y,Kodaira S,Abe O
Cancer Res. 1988 Jul 15;48(14):4053-8
Pubmed id:3962675 Comparison of seven cell lines derived from human gastric carcinomas.
Motoyama T,Hojo H,Watanabe H
Acta Pathol Jpn. 1986 Jan;36(1):65-83
Pubmed id:3095279 Detection of amplified DNA sequences in gastric cancers by a DNA renaturation method in gel.
Nakatani H,Tahara E,Yoshida T,Sakamoto H,Suzuki T,Watanabe H,Sekiguchi M,Kaneko Y,Sakurai M,Terada M
Jpn J Cancer Res. 1986 Sep;77(9):849-53

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LOT Information

Cell No.   JCRB0255 Cell Name   
LOT No.   12152011 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS, GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   7.7 - 10 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Passage twice a week.
Doubling Time   approx. 25 hrs. Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   97.8 Antibiotics Used   free
Passage Number   P21 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0255 Cell Name   
LOT No.   11252008 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. No. 10099, Lot 484375) Temperature   37 C
Cell Density at Seeding   1.4 - 3.3 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Passage twice a week.
Doubling Time   approx. 30 hrs Cell Number in Vial (cells/1ml)   7.6 x 10^6
Viability at cell freezing (%)   97 Antibiotics Used   free
Passage Number   P22 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0255 Cell Name   
LOT No.   08222004 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   5-8 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   ca. 1.4 days Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   77.2 Antibiotics Used   free
Passage Number   P21 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0255 Cell Name   
LOT No.   071299 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   5 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   D.T. = ca 50 hrs Cell Number in Vial (cells/1ml)   4.3 x 10^6
Viability at cell freezing (%)   92 Antibiotics Used   free
Passage Number   P22 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FCS-RPMI1640 CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0255 Cell Name   MKN74
LOT No.   011996 Lot Specification   distribution
Medium   RPMI1640 medium with 10% heat-inactivated fetal bovine serum (JRS CO19407) Temperature   37 C
Cell Density at Seeding   2.0x10^5 cells/ml Methods for Passages   Wash cells once with PBS(-) and treat cells with 0.25% trypsin and 0.02% EDTA for 10-15 min. at 37C.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.6x10^6
Viability at cell freezing (%)   82.0 Antibiotics Used   free
Passage Number   P20 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LDH, NP, G6PD (human confirmed)
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0255 Cell Name   MKN74
LOT No.   031489 Lot Specification   distribution
Medium   RPMI 1640 + FCS 10% Temperature   37
Cell Density at Seeding   1/10 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.2x10^6
Viability at cell freezing (%)   71% Antibiotics Used   free
Passage Number   P15* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LD,G6PD,NP,PepB,AST,MD,MPI
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0255 Cell Name   
LOT No.   042093 Lot Specification   distribution
Medium   RPMI 1640 + FCS 10% Temperature   37
Cell Density at Seeding   1/10 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time    Cell Number in Vial (cells/1ml)   10^6
Viability at cell freezing (%)   NT Antibiotics Used   free, Mycoplasma-eliminated previously by MC-210
Passage Number   *P17 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST, G6PD, LD, MD, MPI, PEPB
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0255 Cell Name   MKN74
LOT No.   08252017 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 25 hrs. Cell Number in Vial (cells/1ml)   2.6 x 10^6
Viability at cell freezing (%)   96.9 Antibiotics Used   free
Passage Number   P21 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   96.9
Additional information   
Cell No.   JCRB0255 Cell Name   MKN74
LOT No.   04092020 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   3.2 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability at cell freezing (%)   98.9 Antibiotics Used   free
Passage Number   P21 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   97.9
Additional information   
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