JCRB0405 HLF

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0405 Cell Name   HLF
Profile   hepatoma, non-differentiated Other Name   HLF
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   liver, gallbladder
Case history    Metastasis   
Tissue Metastasized    Genetics   hepatoma, non-differentiated, age 68, male
Life Span    Crisis PDL   
Morphology    Character   hepatoma, non-differentiated, age 68, male
Classify    Established by   Doi,I. & Sato,J.
Registered by   Sato,J. Regulation for Distribution   
Comment    Year   
Medium   Dulbecco's modified Eagle's medium with with 5% fetal bovine serum. Methods for Passages   Cells are rinsed with PBS(-) and treat with 0.2% trypsin and 0.02% EDTA at room temperature.
Cell Number on Passage   1.5 x 10^4 cells/sq.cm Race   
CO2 Conc.   5 % Tissue Sampling   liver
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2947
Reference
Pubmed id:52570Establishment and some biological characteristics of human hepatoma cell lines.
Dor I,Namba M,Sato J
Gan. 1975 Aug;66(4):385-92

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LOT Information

Cell No.   JCRB0405 Cell Name   
LOT No.   10102012 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with with 5% fetal bovine serum (GIBCO Cat. # 10099). Temperature   37 C
Cell Density at Seeding   5.8 - 14 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 42 hrs Cell Number in Vial (cells/1ml)   8.1 x 10^5
Viability at cell freezing (%)   97.6 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0405 Cell Name   HLF
LOT No.   05242009 Lot Specification   distribution
Medium   DMEM with 5% heat inactivated fetal bovine serum(Hyclone GRD0051) Temperature   37 C
Cell Density at Seeding   1.4x10^2 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.9x10^6
Viability at cell freezing (%)   99.7 Antibiotics Used   free
Passage Number   p7* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11
D13S317:8
D7S820:11
D16S539:9,11
VWA:16
TH01:6,9
AM:X
TPOX:8
CSF1PO:10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0405 Cell Name   
LOT No.   07072005 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 5% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   2 - 3 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 42 hrs Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   Unknown (P7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS -DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0405 Cell Name   HLF
LOT No.   122895 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 20% calf serum (ICN Flow.1059023) Temperature   37 C
Cell Density at Seeding   1.7x10^4 cells/sq.cm. Methods for Passages   Rised cells with PBS(-) once and cells were treated with 0.25% trypsin and 0.02% EDTA at rm temp.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.70x10^6
Viability at cell freezing (%)   97.6 Antibiotics Used   free
Passage Number   P4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0405 Cell Name   
LOT No.   120198 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 5% fetal bovine serum (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   2-3 x 10^4 cells/ml Methods for Passages   0.2% trypsin and 0.02% EDTA
Doubling Time   D.T.=ca. 23 hrs Cell Number in Vial (cells/1ml)   6.4 x 10^5
Viability at cell freezing (%)   88.0 Antibiotics Used   free
Passage Number   P7* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0405 Cell Name   HLF
LOT No.   09102020 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   0.2- 1.0 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   100 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   97.6 Additional information   
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