JCRB0430 KMS-12-PE

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0430 Cell Name   KMS-12-PE
Profile   myeloma, late stage of B-cell differentiation Other Name   KMS-12_PE
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   64
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   myeloma Metastasis   
Tissue Metastasized    Genetics   late stage B-cell differentiation, EBV Ag.negative
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   non-producing, transferrin receptors positive
Classify   transformed Established by   Namba,M. et al.
Registered by   Namba,M. Regulation for Distribution   No commercial purpose.
Comment    Year   
Medium   RPMI1640 medium with 10% fetal bovine serum. Methods for Passages   Simple dilution.
Cell Number on Passage   5x10^5 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   pleural effusion
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1333
Reference
Pubmed id:2768132Establishment of five human myeloma cell lines.
Namba M,Ohtsuki T,Mori M,Togawa A,Wada H,Sugihara T,Yawata Y,Kimoto T
In Vitro Cell Dev Biol. 1989 Aug;25(8):723-9

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LOT Information

Cell No.   JCRB0430 Cell Name   
LOT No.   102396 Lot Specification   distribution
Medium   RPMI1640 medium with 10% FCS (heat-inactivation isrecommended) (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   1-2 x 10^5 cells/ml Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   3.1 x 10^6
Viability at cell freezing (%)   72.7 Antibiotics Used   free
Passage Number   P6* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human by NP, G6PD (type B), MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0430 Cell Name   KMS-12-PE
LOT No.   05012015 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat.# 10091). Temperature   37 C
Cell Density at Seeding   1 - 2 x 10^5 cells/ml Methods for Passages   Simple dilution (suspension culture).
Doubling Time    Cell Number in Vial (cells/1ml)   8.4 x 10^5
Viability at cell freezing (%)   70 Antibiotics Used   free
Passage Number   Unknown (12 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   70
Additional information   
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