JCRB0521 MRC-5-30

Cell information

Important Notice(s)

Notes for the cultivation of human normal diploid cells (JCRB05**) : ヒト正常二倍体細胞の取り扱い上の注意点

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0521 Cell Name   MRC-5-30
Profile   fetal lung, normal diploid fibroblast, PDL=about 30 Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   14 weeks
Identity   available Tissue for Primary Cancer   lung
Case history    Metastasis   
Tissue Metastasized    Genetics   lung,normal diploid fibroblast
Life Span    Crisis PDL   
Morphology    Character   lung,normal diploid fibroblast
Classify   normal Established by   Jacobs,J.P. et al.
Registered by   Ooka,H. Regulation for Distribution   
Comment    Year   1989
Medium   Eagle's minimal essential medium with 10% fetal bovine serum Methods for Passages   Wash in CMF-PBS.Add 0.25 trypsin.Incubation at 37C for 10-15 min.Suspend cells in culture medium.
Cell Number on Passage   sprit ratio=1/4 Race   
CO2 Conc.   5 % Tissue Sampling   fetus lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3027
Reference
Pubmed id:7389832A new human diploid cell strain, TIG-1, for the research on cellular aging.
Ohashi M,Aizawa S,Ooka H,Ohsawa T,Kaji K,Kondo H,Kobayashi T,Noumura T,Matsuo M,Mitsui Y,Murota S,Yamamoto K,Ito H,Shimada H,Utakoji T
Exp Gerontol. 1980;15(2):121-33
Pubmed id:12635218Error theory and ageing in human diploid fibroblasts.
Lewis CM,Tarrant GM
Nature. 1972 Oct 6;239(5371):316-8
Pubmed id:12635262Altered enzymes in ageing human fibroblasts.
Holliday R,Tarrant GM
Nature. 1972 Jul 7;238(5358):26-30
Pubmed id:4316953Characteristics of a human diploid cell designated MRC-5.
Jacobs JP,Jones CM,Baille JP
Nature. 1970 Jul 11;227(5254):168-70

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LOT Information

Cell No.   JCRB0521 Cell Name   
LOT No.   05142008 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat.# 10099, lot 484375). This lot was frozen using DMSO. Temperature   37 C
Cell Density at Seeding   4 - 8 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 40 hrs Cell Number in Vial (cells/1ml)   2.3 x 10^6
Viability at cell freezing (%)   94.5 Antibiotics Used   free
Passage Number   Unknown (3 at bank) PDL   29.5
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0521 Cell Name   
LOT No.   R052 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% FCS This stock was freezed using DMSO. Temperature   37 C
Cell Density at Seeding    Methods for Passages   Treat cells with 0.25% trypsin for 10-15 min. Split ratio, 1:4.
Doubling Time    Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   90.0 Antibiotics Used   100 ug/ml streptmycin and 100 units/ml penicillin
Passage Number    PDL   29
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0521 Cell Name   MRC-5-30
LOT No.   10072013 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. #10099). This lot was frozen using DMSO. Temperature   37C
Cell Density at Seeding   sprit ratio=1/4 (3.3 - 15 x 10^4 cells/mL) Methods for Passages   Wash in CMF-PBS.Add 0.25% trypsin - 0.02% EDTA. Incubation at 37C for 10-15 min.Suspend cells in culture medium.
Doubling Time    Cell Number in Vial (cells/1ml)   1.3 x 10^6 (cells/1m
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   Unknown (3 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB),NP,LDH were examined. Human,not HeLa
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS -EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   93.0%
Additional information   
Cell No.   JCRB0521 Cell Name   MRC-5-30
LOT No.   09112017 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   5.2 - 5.8 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 26 hrs. Cell Number in Vial (cells/1ml)   1.0 x 10^6
Viability at cell freezing (%)   89.5 Antibiotics Used   free
Passage Number   Unknown (3 at bank) PDL   PDL=27.2
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   89.5
Additional information   
Cell No.   JCRB0521 Cell Name   MRC-5-30
LOT No.   05172021 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   2.7 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 32 hrs Cell Number in Vial (cells/1ml)   7.8 x 10^5
Viability at cell freezing (%)   99 Antibiotics Used   free
Passage Number   Unknown (2 at bank) PDL   29
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS -EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   91
Additional information   
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