JCRB0525 TIG-2M-30

Cell information

Important Notice(s)

Notes for the cultivation of human normal diploid cells (JCRB05**) : ヒト正常二倍体細胞の取り扱い上の注意点

本細胞は筋芽細胞ではありません

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0525 Cell Name   TIG-2M-30
Profile   muscular tissue derived normal diploid fibroblast (not myoblast), PDL=about 30 Other Name   TIG-2M
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   13 week fetus
Identity   available Tissue for Primary Cancer   muscular system
Case history   normal Metastasis   
Tissue Metastasized    Genetics   muscular tissue-derived normal diploid fibroblast
Life Span    Crisis PDL   
Morphology   fibroblast-like Character   muscular tissue-derived normal diploid fibroblast
Classify   normal Established by   Tky.Inst.Geront.
Registered by   Ooka,H. Regulation for Distribution   
Comment   Refer attached instruction . Year   1989
Medium   Eagle's minimal essential medium with 10% fetal bovine serum. Methods for Passages   Cells are treated with 0.25% trypsin and 0.02% EDTA when subculturing cells. Subculture every 6 days and change medium 3-4 days after subculture.
Cell Number on Passage   Split ratio=1/4 Race   
CO2 Conc.   5 % Tissue Sampling   muscle
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3204
Reference
Pubmed id:7421303Cell-surface changes accompanying aging in human diploid fibroblasts: effects of tissue, donor age and genotype.
Aizawa S,Mitsui Y,Kurimoto F,Matsuoka K
Mech Ageing Dev. 1980 Jul;13(3):297-307

Images



LOT Information

Cell No.   JCRB0525 Cell Name   
LOT No.   10132009 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat. # 10099). This lot was frozen using DMSO. Temperature   37 C
Cell Density at Seeding   approx. 2 - 3 x 10^4 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin.
Doubling Time   approx. 43 hrs Cell Number in Vial (cells/1ml)   6.6 x 10^5
Viability at cell freezing (%)   91.0 Antibiotics Used   free
Passage Number   Unknown (2 at bank) PDL   42
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0525 Cell Name   
LOT No.   R021 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum. This lot was frozen using DMSO. Temperature   37 C
Cell Density at Seeding   split ratio = 1:4 Methods for Passages   0.25% trypsin in Ca, Mg-free PBS
Doubling Time    Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   93.6 Antibiotics Used   100 units/ml penicillin and 100 ug/ml streptomycin
Passage Number    PDL   37
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0525 Cell Name   TIG-2M-30
LOT No.   11022015 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091). This lot was frozen using DMSO. Temperature   37 C
Cell Density at Seeding   2.0 - 3.3 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 3 days Cell Number in Vial (cells/1ml)   9.4 x 10^5
Viability at cell freezing (%)   92 Antibiotics Used   free
Passage Number   Unknown (3 at bank) PDL   42.7
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   92
Additional information   
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