JCRB0527 TIG-102

Cell information

Important Notice(s)

Notes for the cultivation of human normal diploid cells (JCRB05**) : ヒト正常二倍体細胞の取り扱い上の注意点

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0527 Cell Name   TIG-102
Profile   Normal human skin fibroblast cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   97
Identity   available Tissue for Primary Cancer   skin
Case history   normal Metastasis   
Tissue Metastasized    Genetics   skin, normal diploid fibroblast
Life Span   finite Crisis PDL   
Morphology   fibroblast-like Character   skin, normal diploid fibroblast
Classify   normal Established by   Yamamoto,K.
Registered by   Ooka,H Regulation for Distribution   
Comment   Life span = PD41 Year   1989
Medium   Eagle's minimal essential medium with 10% fetal bovine serum Methods for Passages   Wash in CMF-PBS.Add 0.25% trypsin.Incubate at 37C for 10-15min.Suspend cells in medium.
Cell Number on Passage   6-7x10^4 cells/cm^2 Race   Japanese
CO2 Conc.   5 % Tissue Sampling   skin
Virus Summary    [-/negative, +/positive, nt/not tested,GAPDH is positive control] Virus Tested   GAPDH(+),CMV(-),EBV(-),HHV6(-),HHV7(-),BKV(-),JCV(-),ADV(-),parvoB19(-),HBV(-),HTLV1(-),HTLV2(-),HIV1(-),HIV2(-),HPV18(-)
Tissue Type   normal diploid (2n=46, XX)
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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3187
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LOT Information

Cell No.   JCRB0527 Cell Name   
LOT No.   05232007 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (FBS lot; GIBCO 484375). This lot was frozen using DMSO. Temperature   37 C
Cell Density at Seeding   2-3 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Split ratio = 1:4
Doubling Time   approx. 70 hrs Cell Number in Vial (cells/1ml)   4.3 x 10^4
Viability at cell freezing (%)   68.6 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   28.7
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0527 Cell Name   TIG-102
LOT No.   07072014 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   1.3 - 4.2 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   4.8 x 10^5
Viability at cell freezing (%)   88 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   28.7
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   88
Additional information   
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