JCRB0604 PSV811

Cell information

Important Notice(s)

JCRB0604 PSV811 is derivative from WI-38.

JCRB No.   JCRB0604 Cell Name   PSV811
Profile   Stop providing for the issue of quality control. (Please contact us for more information) This cell line was originally beleived to be derived from werner's syndrom but identified as same as WI38. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   skin
Case history   originally thought to be a werver's syndrome. Metastasis   
Tissue Metastasized    Genetics   skin
Life Span    Crisis PDL   
Morphology    Character   skin, werner's syndrome
Classify   mutant Established by   Matsumura,T.
Registered by   Kuroki,T. Regulation for Distribution   Distribution allowed after papers are published.
Comment    Year   
Medium   Eagle's minimal essential medium with 10% fetal bovine serum Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin
Cell Number on Passage   1-2x10^4 cells/cm^2 Race   Japanese
CO2 Conc.   5 % Tissue Sampling   skin
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3142
Reference
Pubmed id:3002148Studies of SV40-infected Werner syndrome fibroblasts.
Matsumura T,Nagata M,Konishi R,Goto M
Adv Exp Med Biol. 1985;190():313-30
Pubmed id:6301867Decrease in the average size of replicons in a Werner syndrome cell line by Simian virus 40 infection.
Hanaoka F,Takeuchi F,Matsumura T,Goto M,Miyamoto T,Yamada M
Exp Cell Res. 1983 Apr 1;144(2):463-7

Images



LOT Information

Cell No.   JCRB0604 Cell Name   PSV811
LOT No.   110896 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (JRHB 1B1062). Temperature   37 C
Cell Density at Seeding   1-2x10^4 cells/sq.cm. Methods for Passages   Treat cells with 0.04% tripsin in PBS.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.7x10^6
Viability at cell freezing (%)   97.9 Antibiotics Used   free
Passage Number   P3* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0604 Cell Name   
LOT No.   900127 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum Temperature   37
Cell Density at Seeding    Methods for Passages   EDTA , trypsin
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0604 Cell Name   
LOT No.   911204 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum Temperature   37
Cell Density at Seeding    Methods for Passages   EDTA , trypsin
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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