JCRB0611 KATOIII

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0611 Cell Name   KATOIII
Profile   signet ring cell carcinoma Other Name   KATOIII
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   stomach
Case history    Metastasis   
Tissue Metastasized    Genetics   stomach cancer, signet ring cell carcinoma
Life Span    Crisis PDL   
Morphology    Character   stomach cancer, signet ring cell carcinoma
Classify    Established by   Sekiguchi,M.
Registered by   Sekiguchi,M. Regulation for Distribution   
Comment    Year   1985
Medium   RPMI1640 medium 45% with 45% Eagle's minimal essential medium and 10% heat inactivated FBS. Methods for Passages   Simple dilution.
Cell Number on Passage   approx. 2 x 10^5 cells/mL Race   
CO2 Conc.   5 % Tissue Sampling   stomach
Virus Summary   tested Virus Tested   CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(nt) [-/negative, +/positive, nt/not tested]
Tissue Type   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0371
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 907276
Reference
Pubmed id:3095279 Detection of amplified DNA sequences in gastric cancers by a DNA renaturation method in gel.
Nakatani H,Tahara E,Yoshida T,Sakamoto H,Suzuki T,Watanabe H,Sekiguchi M,Kaneko Y,Sakurai M,Terada M
Jpn J Cancer Res. 1986 Sep;77(9):849-53
Pubmed id:209229 Establishment of cultured cell lines derived from a human gastric carcinoma.
Sekiguchi M,Sakakibara K,Fujii G
Jpn J Exp Med. 1978 Feb;48(1):61-8

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LOT Information

Cell No.   JCRB0611 Cell Name   
LOT No.   05232008 Lot Specification   distribution
Medium   1:1 mixture of RPMI 1640 and Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat.# 10099, lot 484375) Temperature   37 C
Cell Density at Seeding   approx. 2 x 10^5 cells/ml Methods for Passages   Dilution. Cultivate as suspension culture. (Some cells are adherant. If needed, adherant cells are harvested by vigorous pipetting or 0.05% trypsin)
Doubling Time   32 hrs Cell Number in Vial (cells/1ml)   4.2 x 10^6
Viability at cell freezing (%)   90.9 Antibiotics Used   free
Passage Number   P715 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0611 Cell Name   
LOT No.   06302003 Lot Specification   distribution
Medium   1:1 mixture of Eagle's MEM and RPMI1640 with heat-inactivated fetal bovine serum Temperature   37 C
Cell Density at Seeding   1.5 x 10^5 cells/ml Methods for Passages   Dilution. Cultivate as suspension culture. Some cells are adherent but these are hardly to detach by pipetting, and not used for subcultivation.
Doubling Time   D.T. = 1.5 days Cell Number in Vial (cells/1ml)   1.3 x 10^6
Viability at cell freezing (%)   95.2 Antibiotics Used   free
Passage Number   P713 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST and LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - basal medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0611 Cell Name   KATOIII
LOT No.   012296 Lot Specification   distribution
Medium   RPMI1640 : Eagle's minimal essential medium = 1 : 1, mixed medium with 10% heat inactivated FBS. Temperature   37 C
Cell Density at Seeding   2.0x10^5 cells/ml Methods for Passages   Simple dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.4x10^6
Viability at cell freezing (%)   94.0 Antibiotics Used   Free
Passage Number   P712 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, LDH, G6PD (human confirmed)
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0611 Cell Name   
LOT No.   062397 Lot Specification   distribution
Medium   1:1 mixture of RPMI1640 medium and Eagle's MEM with 10% heat-inactivated fetal bovine serum Temperature   37 C
Cell Density at Seeding   2 x 10^5 Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   3.0 x 10^6
Viability at cell freezing (%)   91.0 Antibiotics Used   free
Passage Number   P713 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0611 Cell Name   KATOIII
LOT No.   12152014 Lot Specification   distribution
Medium   1:1 mixture of RPMI1640 medium and Eagle's minimum essential medium and 10% fetal bovine serum (FBS; GIBCO Cat. # 10091). Temperature   37 C
Cell Density at Seeding   1.3 - 4.4 x 10^5 cells/mL Methods for Passages   Dilution. Cultivate as suspension culture. (Some cells are adherant. If needed, adherant cells are harvested by vigorous pipetting or 0.07% trypsin)
Doubling Time    Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   84.2 Antibiotics Used   free
Passage Number   P715 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   84.2
Additional information   
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