JCRB0614 NY

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0614 Cell Name   NY
Profile   osteosarcoma, femur Other Name   NY
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   sarcoma
Case history    Metastasis   
Tissue Metastasized    Genetics   osteosarcoma
Life Span    Crisis PDL   
Morphology    Character   osteosarcoma
Classify    Established by   Sekiguchi,M.
Registered by   Sekiguchi,M. Regulation for Distribution   
Comment    Year   1985
Medium   90% MEM + 10% FCS Methods for Passages   rubber policeman
Cell Number on Passage   5x10^4 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   osteosarcoma
Virus Summary   tested Virus Tested   CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(nt) [-/negative, +/positive, nt/not tested]
Tissue Type   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1613
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 910849
Reference
Pubmed id:6596455 A cultured cell line established in vitro from human osteosarcoma.
Sekiguchi M,Asanuma K,Satomura T,Fukushima H,Fujji G,Shimoda T,Fukunaga M,Ishikawa E
Jpn J Exp Med. 1983 Dec;53(6):289-92

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LOT Information

Cell No.   JCRB0614 Cell Name   
LOT No.   091198 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal calf serum Temperature   37 C
Cell Density at Seeding   5-8 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. (Instead, rubber policeman can be used.)
Doubling Time   approx. 60 hrs Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   94.0 Antibiotics Used   free
Passage Number   P10* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0614 Cell Name   NY
LOT No.   09262016 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   2.5 - 3.0 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 30 hrs. Cell Number in Vial (cells/1ml)   2.8 x 10^6
Viability at cell freezing (%)   96 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   96
Additional information   
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