JCRB0618 TGW

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0618 Cell Name   TGW
Profile   neuroblastoma, adrenal gland Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   neural
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology    Character   neuroblastoma
Classify   tumor Established by   Sekiguchi,M.
Registered by   Sekiguchi,M. Regulation for Distribution   
Comment    Year   1989
Medium   Eagle's minimal essential medium with 10% fetal calf serum Methods for Passages   Cells are treated with 0.25% trypsin and 0.02% EDTA at 37 C prior to harvest cells.
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   adrenal gland
Virus Summary   tested Virus Tested   GAPDH(+), CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), HBV(-), parvoB19(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-)[-/negative, +/positive, nt/not tested, GAPDH = positive control]
Tissue Type   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1771
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 910780
Reference
Pubmed id:2335222 Origin of human neuroblastoma cell lines TGW and TNB1.
Tsuchida Y,Sekiguchi M,Kaneko Y,Kanda N
FEBS Lett. 1990 Apr 24;263(2):191

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LOT Information

Cell No.   JCRB0618 Cell Name   
LOT No.   06012007 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (FBS lot; GIBCO 484375) Temperature   37 C
Cell Density at Seeding   1.3-1.8 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Subculture twice a week.
Doubling Time   32 hrs Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   95.2 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0618 Cell Name   TGW
LOT No.   04172014 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal Bovine serum (FBS; GIBCO Cat. # 10091). Temperature   37 C
Cell Density at Seeding   approx. 2 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   D.T. = approx. 27 hrs Cell Number in Vial (cells/1ml)   3.6 x 10^6
Viability at cell freezing (%)   96.0 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   96.0
Additional information   
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