JCRB0732 A9(0439)-1

Cell information

Important Notice(s)

ヒト単染色体導入ハイブリッド細胞の分譲について:鳥取大とのMTA締結が必要です

About the distribution of human monochromosomal hybrid cells: Requirement of MTA between customers and Tottori University

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0732 Cell Name   A9(0439)-1
Profile   contains human chromosome Xpter>Xq26::3p12>3pter (MTA with Tottori Univ. is required.) Other Name   
Animal   human chromosome-introduced Strain   
Genus   Mus Species   musclus
Sex    Age   
Identity    Tissue for Primary Cancer   human chromosomal hybrid
Case history    Metastasis   
Tissue Metastasized    Genetics   contains human chromosome Xpter>Xq26::3p12>3pter
Life Span    Crisis PDL   
Morphology    Character   contains human chromosome Xpter>Xq26::3p12>3pter
Classify    Established by   Oshimura,M.
Registered by   M. Oshimura Regulation for Distribution   MTA with Tottori Univ. is required.
Comment    Year   1988
Medium   Dulbecco's modified Eagle's medium with 10 % fetal calf serum and HAT solution. Methods for Passages   trypsin
Cell Number on Passage   split ratio=1/4 Race   
CO2 Conc.   5 % Tissue Sampling   hybrid
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2825
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LOT Information

Cell No.   JCRB0732 Cell Name   A9(0439)-1
LOT No.   022297 Lot Specification   distribution
Medium   Dubecco's modified Eagle's medium with HAT and 10%fetal bovine serum (JRSientific DO10400). Temperature   37 C
Cell Density at Seeding   1.0 x 10^4 cells/sq.cm. Methods for Passages   Cells harvested with 0.25% trypsin and 0.02% EDTA (37 C,1 min.).
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.3x10^6
Viability at cell freezing (%)   94.0 Antibiotics Used   free
Passage Number   P3* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   examined
Chromosome Mode    Chromosome Information   examined
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO but omitted HAT solution. CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
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