JCRB0742 SR-3Y1-2

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0742 Cell Name   SR-3Y1-2
Profile   3Y1 derivative, transformed by Rous sarcoma virus, (Fischer rat) Other Name   
Animal   rat Strain   Fischer
Genus   Rattus Species   norvegicus
Sex   F Age   
Identity    Tissue for Primary Cancer   embryo
Case history   3Y1 transformed by virus Metastasis   
Tissue Metastasized    Genetics   whole embryo,transformed 3Y1 by Rous sarcoma virus
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   whole embryo,transformed 3Y1 by Rous sarcoma virus
Classify   transformed Established by   Kimura,G.
Registered by   Kimura,G. Regulation for Distribution   Citation of #966 is required.
Comment    Year   1988
Medium   Dulbecco's modified Eagle's medium with 0.1% glucose and 10% fetal calf serum. Methods for Passages   Cells treated with 0.05% trypsin and 5mM EDTA when subculture. Dilution 1/10.
Cell Number on Passage   Below 1x10^6 cells/50mm dish. Race   
CO2 Conc.   5 % Tissue Sampling   embryo/fetus whole
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_8116
Pubmed id:2844831Influence of the deprivation of a single amino acid on cellular proliferation and survival in rat 3Y1 fibroblasts and their derivatives transformed by a wide variety of agents.
Tanaka H,Zaitsu H,Onodera K,Kimura G
J Cell Physiol. 1988 Sep;136(3):421-30
Pubmed id:680001A temperature-sensitive mammalian cell mutant exhibiting micronucleation.
Sato K,Hama-Inaba H
Exp Cell Res. 1978 Jul;114(2):484-6


LOT Information

Cell No.   JCRB0742 Cell Name   
LOT No.   092299 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   2 - 5 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   D.T. = 22 - 25 hrs Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   91 Antibiotics Used   free
Passage Number   P6* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as rat by NP, G6PD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information