JCRB0801 Caki-1

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0801 Cell Name   Caki-1
Profile   renal clear cell carcinoma Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   49
Identity   available Tissue for Primary Cancer   kidney
Case history    Metastasis   
Tissue Metastasized    Genetics   renal cancer
Life Span    Crisis PDL   
Morphology    Character   renal cancer
Classify    Established by   Fogh, J.
Registered by   Akiyama, M. Regulation for Distribution   
Comment    Year   1986
Medium   Eagle's minimal essential medium with 10% fetal calf serum. Methods for Passages   Treat cells with 0.25% trypsin & dilute in fresh medium.Change medium twice a week.
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   renal
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0234
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 905963
Reference
Pubmed id:327080One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.
Fogh J,Fogh JM,Orfeo T
J Natl Cancer Inst. 1977 Jul;59(1):221-6
Pubmed id:833871Absence of HeLa cell contamination in 169 cell lines derived from human tumors.
Fogh J,Wright WC,Loveless JD
J Natl Cancer Inst. 1977 Feb;58(2):209-14

Images



LOT Information

Cell No.   JCRB0801 Cell Name   
LOT No.   07142008 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat.# 10099, lot 484375) Temperature   37 C
Cell Density at Seeding   approx. 4 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Subculture twice every week at split ratio 1:4.
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   80.4 Antibiotics Used   free
Passage Number   P54 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0801 Cell Name   Caki-1
LOT No.   03262008 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% heat inactivated fetal bovine serum(Intergen RB52305) Temperature   37 C
Cell Density at Seeding   1.3x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.5x10^6
Viability at cell freezing (%)   99.6 Antibiotics Used   NT
Passage Number   p52 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0801 Cell Name   
LOT No.   092195 Lot Specification   distribution
Medium   MEM + 10% FCS Temperature   37
Cell Density at Seeding   split ratio=1:6 Methods for Passages   0.25% trypsin. This cell line grows very slowly. Change medium twice a week.
Doubling Time   about 1 week Cell Number in Vial (cells/1ml)   ca3-5x10^6
Viability at cell freezing (%)    Antibiotics Used   penicillin/streptmycin
Passage Number   P40 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0801 Cell Name   
LOT No.   092298 Lot Specification   distribution
Medium   Eagle's minimal essentiatl medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   5-7 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   65-70 hrs Cell Number in Vial (cells/1ml)   4.2 x 10^5
Viability at cell freezing (%)   80.0 Antibiotics Used   free
Passage Number   P53 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0801 Cell Name   Caki-1
LOT No.   03132015 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   1.0 - 4.5 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 28 hrs Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   87.6 Antibiotics Used   free
Passage Number   P53 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11,12
D13S317:11,12
D7S820:8,12
D16S539:12
VWA:15,17
TH01:6,8
AM:X
TPOX:8,11
CSF1PO:10,11
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   87.6
Additional information   
Cell No.   JCRB0801 Cell Name   Caki-1
LOT No.   07032020 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.9 - 3.1 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 42 hrs. Cell Number in Vial (cells/1ml)   7.4 x 10^5
Viability at cell freezing (%)   99 Antibiotics Used   free
Passage Number   P55 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   99
Additional information   
CLOSE