JCRB0812 RERF-LC-MA

Cell information

Important Notice(s)

JCRB0812 RERF-LC-MA細胞の由来について

JCRB0812 RERF-LC-MA: the problem of origin

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0812 Cell Name   RERF-LC-MA
Profile   Stop providing for the issue of quality control. (Please contact us for more information) small cell carcinoma, exhibits the same DNA profile as lung squamous carcinoma cell line SK-MES-1. (distribution is suspended) Other Name   Matsushima
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   lung (cancer)
Case history   small cell carcinoma Metastasis   
Tissue Metastasized    Genetics   
Life Span    Crisis PDL   
Morphology    Character   
Classify    Established by   Kohno,N. & Yokosaki.Y.
Registered by   Akiyama,M. Regulation for Distribution   
Comment    Year   1987
Medium   Eagle's minimal essential medium with 10% fetal calf serum Methods for Passages   0.25% trypsin and dilute in fresh medium. Change medium twice a week
Cell Number on Passage   split ratio=1/6 Race   
CO2 Conc.   5 % Tissue Sampling   lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3153
Reference
Pubmed id:1330284In vitro measurement of chemosensitivity of human small cell lung and gastric cancer cell lines toward cell cycle phase-nonspecific agents under the clinically equivalent area under the curve.
Mitsuhashi Y,Inaba M,Sugiyama Y,Kobayashi T
Cancer. 1992 Nov 15;70(10):2540-6

Images



LOT Information

Cell No.   JCRB0812 Cell Name   
LOT No.   01252008 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS lot; GIBCO 484375) Temperature   37 C
Cell Density at Seeding   2 - 3 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 1.5 days Cell Number in Vial (cells/1ml)   1.0 x 10^6
Viability at cell freezing (%)   80 Antibiotics Used   free
Passage Number   P34 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0812 Cell Name   
LOT No.   07172007 Lot Specification   distribution
Medium   Eagle's minimam essential medium with 10% fetal bovine serum (FBS lot; GIBCO 484375) Temperature   37 C
Cell Density at Seeding   2-4 x 10^4 cells/ml Methods for Passages   0.25% trypsin
Doubling Time   approx. 1.5 days Cell Number in Vial (cells/1ml)   9.1 x 10^5
Viability at cell freezing (%)   95.4 Antibiotics Used   free
Passage Number   P33 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0812 Cell Name   RERF-LC-MA
LOT No.   091498 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (lot#.Summit 8.1E+41). Temperature   37 C
Cell Density at Seeding   2.0x10^4 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.5x10^6
Viability at cell freezing (%)   89.1 Antibiotics Used   free
Passage Number   P35 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(type B), LD and NP examined. Human.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11
D13S317:11
D7S820:8
D16S539:13,14
VWA:14
TH01:6,10
AM:XY
TPOX:8
CSF1PO:12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0812 Cell Name   
LOT No.   081495 Lot Specification   distribution
Medium   MEM +10%FCS Temperature   37
Cell Density at Seeding   split ratio=1:6 Methods for Passages   0.25% trypsin and dilute in fresh medium. Change medium twice a week.
Doubling Time    Cell Number in Vial (cells/1ml)   ca3-5x10^6
Viability at cell freezing (%)    Antibiotics Used   penicillin/streptomycin
Passage Number   P26 PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   confirmed as human
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0812 Cell Name   
LOT No.   091295 Lot Specification   distribution
Medium   MEM + 10%FCS Temperature   37
Cell Density at Seeding   split ratio=1:6 Methods for Passages   0.25% trypsin and dilute in fresh medium. Change medium twice a week
Doubling Time    Cell Number in Vial (cells/1ml)   3-5x10^6
Viability at cell freezing (%)    Antibiotics Used   penicillin/stroptomycin
Passage Number   P25 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
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