JCRB0815 ABC-1

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0815 Cell Name   ABC-1
Profile   adenocarcinoma Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   47
Identity   available Tissue for Primary Cancer   lung (cancer)
Case history    Metastasis   
Tissue Metastasized    Genetics   adenocarcinoma
Life Span    Crisis PDL   
Morphology    Character   adenocarcinoma
Classify    Established by   Hiraki,S.
Registered by   Akiyama,M. Regulation for Distribution   
Comment    Year   1987
Medium   Eagle's minimal essential medium with 10% fetal calf serum Methods for Passages   0.25% trypsin and dilute in fresh medium. Change medium twice a week
Cell Number on Passage   split ratio=1/6 Race   
CO2 Conc.   5 % Tissue Sampling   lung
Virus Summary   tested Virus Tested   CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-) [-/negative, +/positive]
Tissue Type   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1066
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 906791
Reference
Pubmed id:1752082 The spread of human lung cancer cells on collagens and its inhibition by type III collagen.
Hirai K,Shimada H,Ogawa T,Taji S
Clin Exp Metastasis. 1991 Nov-Dec;9(6):517-27

Images



LOT Information

Cell No.   JCRB0815 Cell Name   ABC-1
LOT No.   02282012 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS, GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   1 - 2 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 52 hrs. Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability (%)   91.4 Antibiotics Used   free
Passage Number   P40 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Additional information   
Cell No.   JCRB0815 Cell Name   ABC-1
LOT No.   07052010 Lot Specification   distribution
Medium   Eagle's minimum essential medium(Nissui) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   2.89-5.58x10^4cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and dilute in fresh medium.Change medium twice a week .Split ratio=1/6
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.47x10^6
Viability (%)   90.8 Antibiotics Used   free
Passage Number   p39 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12
D13S317:8
D7S820:10,12
D16S539:9,12
VWA:16
TH01:10
AM:X
TPOX:8,11
CSF1PO:12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Additional information   
Images
Cell No.   JCRB0815 Cell Name   
LOT No.   12222006 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   1 - 2 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability (%)   98 Antibiotics Used   free
Passage Number   P39 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B)MD, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Additional information   
Cell No.   JCRB0815 Cell Name   
LOT No.   090695 Lot Specification   distribution
Medium   MEM + 10%FCS Temperature   37
Cell Density at Seeding    Methods for Passages   0.25% trypsin and dilute in fresh medium. Change medium twice a week.
Doubling Time    Cell Number in Vial (cells/1ml)   ca3-5x10^6
Viability (%)    Antibiotics Used   penicillin/streptomycin
Passage Number   P35 (BM3) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Additional information   
Cell No.   JCRB0815 Cell Name   
LOT No.   092598 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   2-4 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   53 hrs Cell Number in Vial (cells/1ml)   4.4 x 10^6
Viability (%)   96.0 Antibiotics Used   free
Passage Number   P40 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Additional information   
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