JCRB0830 PEER

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0830 Cell Name   PEER
Profile   Human lymphoid cell line from a case of T-leukemia lacks some typical cell surface properties of T cells. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   4
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   diagnosed as having acute lymphocytic leukemia, T cell type. Presenting symptoms when diagnosed were, massive lymphoadenopaty, hepatosplenomegaly a mediastinal mass. White blood cell count was 1700,000/mm^3 with 99% lymphoblasts. Metastasis   
Tissue Metastasized    Genetics   Negative for B cell markers, and EBV(EBNA).
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   acute lymphocytic leukemia from a case of T cell
Classify   tumor Established by   Ravid,Z. et al.
Registered by   Akiyama,M. Regulation for Distribution   
Comment   Cells originally contaminated with mycoplasmas and decontaminated MC210 in RERF. Year   1989
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   Dilution.
Cell Number on Passage   4 x 10^5 cells/sq.cm. Race   
CO2 Conc.   5 % Tissue Sampling   peripheral blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1913
Reference
Pubmed id:14768698 Establishment and characterization of a new leukaemic T-cell line (Peer) with an unusual phenotype.
Ravid Z,Goldblum N,Zaizov R,Schlesinger M,Kertes T,Minowada J,Verbi W,Greaves M
Int J Cancer. 1980 Jun 15;25(6):705-10

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LOT Information

Cell No.   JCRB0830 Cell Name   
LOT No.   06202003 Lot Specification   distribution
Medium   RPMI1640 medium with 10 - 20% fetal bovine serum (FBS lot; BioWhittaker 0S069F) Temperature   37 C
Cell Density at Seeding   1.1-1.2 x 10^5 cells/ml Methods for Passages   dilution
Doubling Time   D.T. = 40-50 hrs Cell Number in Vial (cells/1ml)   9.5 x 10^5
Viability at cell freezing (%)   87 Antibiotics Used   free
Passage Number   P26 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD and LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0830 Cell Name   
LOT No.   090895 Lot Specification   distribution
Medium   RPMI 1640 Temperature   37
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0830 Cell Name   
LOT No.   091195 Lot Specification   distribution
Medium   RPMI 1640 Temperature   37
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0830 Cell Name   PEER
LOT No.   10012019 Lot Specification   distribution
Medium   RPMI 1640 medium with 10-20% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   0.7 - 3.5 x 10^5 cells/mL Methods for Passages   Simple dilution. The size of cells is small and threfore 1200 rpm x 5 min will be required for collecting cells by centrifugation.
Doubling Time   approx. 39 hrs Cell Number in Vial (cells/1ml)   8.6 x 10^6
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   P25 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   91.2
Additional information   
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