JCRB0833 NH-12

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0833 Cell Name   NH-12
Profile   neuroblastoma, adrenal gland Other Name   neuroblastoma-12
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   1year-3months
Identity   available Tissue for Primary Cancer   neural
Case history   neuroblastoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   neroblastoma cell line
Classify   tumor Established by   Hiyama,E. & Nakamura,N.
Registered by   Akiyama,M. Regulation for Distribution   
Comment    Year   1992
Medium   MEM-alpha with 10% fetal calf serum. Methods for Passages   Cells are treated with 0.25% trypsin and 0.02% EDTA and dilute in fresh medium.
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   adrenal gland
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1605
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 908447
Reference
Pubmed id:1555997Length of telomeric repeats in neuroblastoma: correlation with prognosis and other biological characteristics.
Hiyama E,Hiyama K,Yokoyama T,Ichikawa T,Matsuura Y
Jpn J Cancer Res. 1992 Feb;83(2):159-64

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LOT Information

Cell No.   JCRB0833 Cell Name   NH-12
LOT No.   073197 Lot Specification   distribution
Medium   Alpha MEM with 10% fetal calf serum (JRH bio, lot.4B2013). Temperature   37 C
Cell Density at Seeding   4 x 10^4 cells/sq.cm. Methods for Passages   Harvest cells with 0.25% trypsin and 0.02% EDTA for 5 minutes at room temperature.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.8x10^6
Viability at cell freezing (%)   99.0 Antibiotics Used   free
Passage Number   P54 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0833 Cell Name   NH-12
LOT No.   041097 Lot Specification   distribution
Medium   ****************************** Temperature   
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA   + Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:10,11
D13S317:8,9
D7S820:11,13
D16S539:11
VWA:17,18
TH01:9,10
AM:XY
TPOX:8
CSF1PO:11,12
Adhesion    Exoteric Gene   
Medium for Freezing   ****************************** CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0833 Cell Name   
LOT No.   090595 Lot Specification   distribution
Medium   alpha-MEM + FCS 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   0.02% EDTA + 0.25 % trypsin
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0833 Cell Name   
LOT No.   090695 Lot Specification   distribution
Medium   alpha-MEM + FCS 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   0.02% EDTA + 0.25 % trypsin
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0833 Cell Name   NH-12
LOT No.   10242018 Lot Specification   distribution
Medium   Alpha-MEM with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   0.9 - 2.1 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 48 hrs. Cell Number in Vial (cells/1ml)   3.1 x 10^6
Viability at cell freezing (%)   90.4 Antibiotics Used   free
Passage Number   P58 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - alpha-MEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   78.8
Additional information   
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