JCRB1002 K562/ADM

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1002 Cell Name   K562/ADM
Profile   Adriamycine resistant derivative of K562 cell line. Other Name   K562/ADM
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   53
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history    Metastasis   
Tissue Metastasized    Genetics   chronic myelogenous leukemia, adriamycin resistant
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   Chronic myelogenous leukemia cell line, resistant to adriamycin (300ng/ml), induction of p-glycoprotein by addition of sodium butyrate (10ng/ml).
Classify   mutant Established by   Tsuruo, T.
Registered by   Tsuruo, T. Regulation for Distribution   
Comment   Mycoplasma decontaminated by Tsuruo at 1987. Multi registancy for antibiotics in cultured cell line derived from human. Year   1987
Medium   RPMI1640 medium with 10% fetal calf serum. Methods for Passages   Dilution.
Cell Number on Passage   2x10^5 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   pleural effusion
Virus Summary   tested Virus Tested   CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-) [-/negative, +/positive, nt/not tested]
Tissue Type   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3827
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LOT Information

Cell No.   JCRB1002 Cell Name   K562/ADM
LOT No.   02202002 Lot Specification   distribution
Medium   RPMI1640 medium with 10% heat inactivated fetal calf serum. Temperature   37 C
Cell Density at Seeding   1.7 x 10^5 cells/ml Methods for Passages   Simple dilution.
Doubling Time   Td = 22 hrs Cell Number in Vial (cells/1ml)   2.0 x 10^6
Viability at cell freezing (%)   99.0 Antibiotics Used   free
Passage Number   P4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), NP, LDH examined. Human, not HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:8
D7S820:9,11
D16S539:11,12
VWA:16
TH01:10
AM:X
TPOX:8,9
CSF1PO:9,10
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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Cell No.   JCRB1002 Cell Name   K562/ADM
LOT No.   01312014 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091). Temperature   37 C
Cell Density at Seeding   1.3 - 2.5 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture)
Doubling Time    Cell Number in Vial (cells/1ml)   4.2 x 10^6
Viability at cell freezing (%)   89.1 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   89.1
Additional information   
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