JCRB1003 Kasumi-1

Cell information

Important Notice(s)

On the particles appear in cultures of Kasumi-series cell lines

Surface antigen expressions in Kasumi-1 cells.

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1003 Cell Name   Kasumi-1
Profile   A novel leukemia cell line with an t8;21 chromosome translocation established from a patient attaked by acute myeloid leukemia(AML). Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   7
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   aculte myeloid leukemia (AML). Metastasis   
Tissue Metastasized    Genetics   t(8:21), AML-ETO fusion gene
Life Span   infinite Crisis PDL   
Morphology   myeloblast Character   MPO+, CD34+, CD13+
Classify   tumor Established by   Asou,H.
Registered by   Kyoizumi,S. &Asou,H. Regulation for Distribution   free
Comment    Year   2000
Medium   RPMI1640 medium with 10% heat inactivated fetal calf serum. Methods for Passages   Subculture twice a week at split ratio of 1/2 -1/3 by simple dilution. Suspension cell culture.
Cell Number on Passage   Split ratio = 1/2-1/3. Race   Japanese
CO2 Conc.   5 % Tissue Sampling   blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0589
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 907275
Reference
Pubmed id:11283671 AML1-ETO downregulates the granulocytic differentiation factor C/EBPalpha in t(8;21) myeloid leukemia.
Pabst T,Mueller BU,Harakawa N,Schoch C,Haferlach T,Behre G,Hiddemann W,Zhang DE,Tenen DG
Nat Med. 2001 Apr;7(4):444-51
Pubmed id:8334990 The t(8;21) translocation in acute myeloid leukemia results in production of an AML1-MTG8 fusion transcript.
Miyoshi H,Kozu T,Shimizu K,Enomoto K,Maseki N,Kaneko Y,Kamada N,Ohki M
EMBO J. 1993 Jul;12(7):2715-21
Pubmed id:1720541 t(8;21) breakpoints on chromosome 21 in acute myeloid leukemia are clustered within a limited region of a single gene, AML1.
Miyoshi H,Shimizu K,Kozu T,Maseki N,Kaneko Y,Ohki M
Proc Natl Acad Sci U S A. 1991 Dec 1;88(23):10431-4
Pubmed id:2018839 Establishment of a human acute myeloid leukemia cell line (Kasumi-1) with 8;21 chromosome translocation.
Asou H,Tashiro S,Hamamoto K,Otsuji A,Kita K,Kamada N
Blood. 1991 May 1;77(9):2031-6


Research results by users. Click!
Pubmed id:32180206 Kasumi leukemia cell lines: characterization of tumor genomes with ethnic origin and scales of genomic alterations.
Kasai F,Asou H,Ozawa M,Kobayashi K,Kuramitsu H,Satoh M,Kohara A,Kaneko Y,Kawamura M
Hum Cell. 2020 Jul;33(3):868-876

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LOT Information

Cell No.   JCRB1003 Cell Name   Kasumi-1
LOT No.   03232010 Lot Specification   distribution
Medium   RPMI1640 medium with 10% heat-inactivated fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   4 - 10 x 10^4 cells/ml Methods for Passages   Simple dilution (suspension culture)
Doubling Time   approx. 4-5 days. Cell Number in Vial (cells/1ml)   7.3 x 10^5
Viability at cell freezing (%)   89.1 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   On vial: lot number is mis-labeled as 01023230, correctly 03232010.
Cell No.   JCRB1003 Cell Name   
LOT No.   02262002 Lot Specification   distribution
Medium   RPMI1640 medium with 10% heat inactivated fetal calf serum. (Use 20% serum at thawing) Temperature   37 C
Cell Density at Seeding   Split ratio = 1/2-1/3. Methods for Passages   Subculture twice a week at split ratio of 1/2 -1/3 by simple dilution. Suspension cell culture.
Doubling Time    Cell Number in Vial (cells/1ml)   >10^6
Viability at cell freezing (%)   NT Antibiotics Used   fee
Passage Number    PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   5% DMSO-Culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB1003 Cell Name   Kasumi-1
LOT No.   09032015 Lot Specification   distribution
Medium   RPMI1640 medium with 10% heat-inactivated fetal bovine serum (FBS; GIBCO Cat. # 10091). Temperature   37 C
Cell Density at Seeding   3.1 - 4.9 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture), split ratio of 1/2 -1/3 is recommended.
Doubling Time   approx. 3 days Cell Number in Vial (cells/1ml)   2.4 x 10^6
Viability at cell freezing (%)   72.2 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% heat-inactivated FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   72.2
Additional information   
Cell No.   JCRB1003 Cell Name   Kasumi-1
LOT No.   02092018 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   2.8-5.2x10^5 cells/sq.cm Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.0x10^6
Viability at cell freezing (%)   97.40 Antibiotics Used   free
Passage Number   p8* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   NT
Additional information   Start culture under 2 ml growth medium,because cell number is under 1.0x10^6 cells/vial.
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Cell No.   JCRB1003 Cell Name   Kasumi-1
LOT No.   05112018 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   3.0-4.3x10^5 cells/sq.cm Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.8x10^6
Viability at cell freezing (%)   97.10 Antibiotics Used   free
Passage Number   p8* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:9,11
D13S317:11,13
D7S820:8,11
D16S539:9,12
VWA:14
TH01:6,9
AM:X
TPOX:8,9
CSF1PO:10,12
Adhesion   No Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   80
Additional information   
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