JCRB1009 RERF-GC-1B

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1009 Cell Name   RERF-GC-1B
Profile   Human gastric cancer cell line established from a metastatic tissue of pyloric lymph nodes. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   41
Identity   available Tissue for Primary Cancer   stomach
Case history   gastric cancer Metastasis   Yes
Tissue Metastasized   pyloric lymph nodes Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Gastric adenocarcinoma cell line established from metastatic tumor tissue of pyloric lymph nodes.
Classify   tumor Established by   Kyoizumi,S.
Registered by   Kyoizumi,S. Regulation for Distribution   
Comment   No reference informed when deposited. Year   2001
Medium   RPMI1640 medium with 10% heat inactivated fetal calf serum. Methods for Passages   Cells are harvested after treatment with 0.05% trypsin and 0.02% EDTA at 37 C for 5 min. Subculture once two weeks.
Cell Number on Passage   split ratio = 1/4 Race   Japanese
CO2 Conc.   5 % Tissue Sampling   stomach
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3152
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LOT Information

Cell No.   JCRB1009 Cell Name   RERF-GC-1B
LOT No.   10292008 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099, lot 484375) Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/mL Methods for Passages   0.05% trypsin and 0.02% EDTA. Passage approx. once a week.
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   3.5 x 10^5
Viability at cell freezing (%)   94.2 Antibiotics Used   free
Passage Number   P23 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   Lot no. is labeled as 20081029.
Cell No.   JCRB1009 Cell Name   RERF-GC-1B
LOT No.   07192016 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   3.3 x 4.2 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.05% trypsin and 0.02% EDTA.
Doubling Time   49 hrs Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   95 Antibiotics Used   free
Passage Number   P21 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   95
Additional information   The cell name is mislabeled on vials. The correct cell name is RERF-GC-1B.
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