JCRB1031 JHH-7

Cell information

Important Notice(s)

JCRB1031 JHH-7: HBV Integration

On the agreement for distribution of JHH series cell lines, NOZ and OZ

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1031 Cell Name   JHH-7
Profile   HBV integrated human hepato cellular carcinoma cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   53
Identity   available Tissue for Primary Cancer   liver, gallbladder
Case history   HBs-Ag positive hepato cellular carcinoma (Ed.III) with liver cirrhosis. HBs-Ag(+), HBs-Ab(-), HBe-Ag(-), HBe-Ab(+), HBc-Ab(+), AFP positive (110,000 ng/ml). Metastasis   
Tissue Metastasized    Genetics   HBV-DNA integration observed. 6.0/2.5Kb bands by HindIII were observed.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   
Classify   tumor Established by   Honma,S. Fujise,K. Nagamori,S.
Registered by   Nagamori,S. Regulation for Distribution   Require negotiation and agreement from Dr.Nagamori before shipping.
Comment   Cell Bank will handle to get the approval but the depositor may contact to the requested person directly. Year   2001
Medium   William's E medium with 10% fetal calf serum. Methods for Passages   Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Cell Number on Passage   Not defined. Race   Japanese
CO2 Conc.   5 % Tissue Sampling   liver
Virus Summary   tested Virus Tested   CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), parvoB19(-), HBV(+), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(nt) [-/negative, +/positive, nt/not tested]
Tissue Type   hepato cellular carcinoma
Link
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 1240160
Reference
Pubmed id:1701409 Integration of hepatitis B virus DNA into cells of six established human hepatocellular carcinoma cell lines.
Fujise K,Nagamori S,Hasumura S,Homma S,Sujino H,Matsuura T,Shimizu K,Niiya M,Kameda H,Fujita K
Hepatogastroenterology. 1990 Oct;37(5):457-60
Pubmed id:2484807 [Protein secretion of human cultured liver cells].
Nagamori S,Fujise K,Hasumura S,Homma S,Sujino H,Matsuura T,Shimizu K,Niiya M,Kameda H
Hum Cell. 1988 Dec;1(4):382-90


Research results by users. Click!
Pubmed id:22309595 Fibroblast growth factor 19 expression correlates with tumor progression and poorer prognosis of hepatocellular carcinoma.
Miura S,Mitsuhashi N,Shimizu H,Kimura F,Yoshidome H,Otsuka M,Kato A,Shida T,Okamura D,Miyazaki M
BMC Cancer. 2012 Feb 6;12():56
Pubmed id:21725613 Growth inhibition by NVP-BEZ235, a dual PI3K/mTOR inhibitor, in hepatocellular carcinoma cell lines.
Masuda M,Shimomura M,Kobayashi K,Kojima S,Nakatsura T
Oncol Rep. 2011 Nov;26(5):1273-9
Pubmed id:20044606 Infrequent amplification of JUN in hepatocellular carcinoma.
Endo M,Yasui K,Nakajima T,Gen Y,Tsuji K,Dohi O,Zen K,Mitsuyoshi H,Minami M,Itoh Y,Taniwaki M,Tanaka S,Arii S,Okanoue T,Yoshikawa T
Anticancer Res. 2009 Dec;29(12):4989-94
Pubmed id:19142034 Contribution of AP-1 interference induced by TAC-101 to tumor growth suppression in a hepatocellular carcinoma model.
Eshima K,Fukaya S,Sugimoto A,Mori T,Yokoi H,Yamamoto Y,Sugiura S,Honda S,Masuko N,Murakami K,Yamasaki Y,Kagechika H
Tumour Biol. 2009;30(1):1-7
Pubmed id:16160184 Formation of vesicular stomatitis virus pseudotypes bearing surface proteins of hepatitis B virus.
Saha MN,Tanaka A,Jinno-Oue A,Shimizu N,Tamura K,Shinagawa M,Chiba J,Hoshino H
J Virol. 2005 Oct;79(19):12566-74
Pubmed id:15349899 Proteomic signature corresponding to alpha fetoprotein expression in liver cancer cells.
Yokoo H,Kondo T,Fujii K,Yamada T,Todo S,Hirohashi S
Hepatology. 2004 Sep;40(3):609-17
Pubmed id:11983450 Acyclic retinoid induces partial differentiation, down-regulates telomerase reverse transcriptase mRNA expression and telomerase activity, and induces apoptosis in human hepatoma-derived cell lines.
Yasuda I,Shiratori Y,Adachi S,Obora A,Takemura M,Okuno M,Shidoji Y,Seishima M,Muto Y,Moriwaki H
J Hepatol. 2002 May;36(5):660-71
Pubmed id:12395321 Synergistic induction of apoptosis by acyclic retinoid and interferon-beta in human hepatocellular carcinoma cells.
Obora A,Shiratori Y,Okuno M,Adachi S,Takano Y,Matsushima-Nishiwaki R,Yasuda I,Yamada Y,Akita K,Sano T,Shimada J,Kojima S,Okano Y,Friedman SL,Moriwaki H
Hepatology. 2002 Nov;36(5):1115-24
Pubmed id:10766424 Involvement of insulin-like growth factor binding protein-3 in the retinoic acid receptor-alpha-mediated inhibition of hepatocellular carcinoma cell proliferation.
Murakami K,Matsuura T,Hasumura S,Nagamori S,Yamada Y,Saiki I
Cancer Lett. 2000 Apr 3;151(1):63-70
Pubmed id:10933057 Invasiveness of hepatocellular carcinoma cell lines: contribution of membrane-type 1 matrix metalloproteinase.
Murakami K,Sakukawa R,Ikeda T,Matsuura T,Hasumura S,Nagamori S,Yamada Y,Saiki I
Neoplasia. 1999 Nov;1(5):424-30
Pubmed id:9446792 Cyclin E overexpression responsible for growth of human hepatic tumors with p21WAF1/CIP1/SDI1.
Tsuji T,Miyazaki M,Fushimi K,Mihara K,Inoue Y,Ohashi R,Ohtsubo M,Hamazaki K,Furusako S,Namba M
Biochem Biophys Res Commun. 1998 Jan 14;242(2):317-21
Pubmed id:9932610 4-[3,5-Bis(trimethylsilyl)benzamido] benzoic acid (TAC-101) inhibits the intrahepatic spread of hepatocellular carcinoma and prolongs the life-span of tumor-bearing animals.
Murakami K,Matsuura T,Sano M,Hashimoto A,Yonekura K,Sakukawa R,Yamada Y,Saiki I
Clin Exp Metastasis. 1998 Oct;16(7):633-43
Pubmed id:8835345 Persistence of hepatitis C virus RNA in established human hepatocellular carcinoma cell lines.
Tsuboi S,Nagamori S,Miyazaki M,Mihara K,Fukaya K,Teruya K,Kosaka T,Tsuji T,Namba M
J Med Virol. 1996 Feb;48(2):133-40
Pubmed id:7524639 [A new liver support system composed of functional human cells and a radial-flow bioreactor].
Kawada M,Nagamori S,Aizaki H,Fukaya K,Niiya M,Matsuura T,Sujino H,Hasumura S,Shimizu K,Yoshida H
Hum Cell. 1994 Jun;7(2):95-100
Pubmed id:1707654 [Establishment and characterization of a human hepatocellular carcinoma cell line JHH-7 producing alpha -fetoprotein and carcinoembryonic antigen--changes in secretion of AFP and CEA from JHH-7 cells after heat treatment].
Homma S,Nagamori S,Fujise K,Hasumura S,Sujino H,Matsuura T,Shimizu K,Niiya M,Kameda H
Hum Cell. 1990 Jun;3(2):152-7
Pubmed id:2174476 [Analysis for the integrated hepatitis B virus genome in cells of established human hepatocellular carcinoma cell line JHH-7].
Fujise K,Nagamori S,Hasumura S,Homma S,Fujita K,Sujino H,Matsuura T,Shimizu K,Niiya M,Ohno T
Nihon Shokakibyo Gakkai Zasshi. 1990 Sep;87(9):1851-5
Pubmed id:1701409 Integration of hepatitis B virus DNA into cells of six established human hepatocellular carcinoma cell lines.
Fujise K,Nagamori S,Hasumura S,Homma S,Sujino H,Matsuura T,Shimizu K,Niiya M,Kameda H,Fujita K
Hepatogastroenterology. 1990 Oct;37(5):457-60
Pubmed id:2856500 [Effects of TNF on human hepatocellular carcinoma cell lines and their modification by hyperthermia].
Hasumura S,Nagamori S,Fujise K,Homma S,Sujino H,Matsuura T,Shimizu K,Niiya M,Kameda H,Satomi N
Hum Cell. 1988 Jun;1(2):238-44
Pubmed id:none ヒト肝癌細胞株に対するTumor necrosis factorの影響
蓮村哲 永森静志 藤瀬清隆 本間定 筋野甫 松浦知和 清水恵一郎 新谷稔 里見信子
医学のあゆみ 143(8), 655-656 (1987)
Pubmed id:none 樹立ヒト肝癌株細胞中のB型肝炎ウイルスゲノム
藤瀬 清隆, 藤多 和信, 永森 静志, 蓮村 哲, 本間 定, 筋野 甫, 松浦 知和, 清水 恵一郎, 新谷 稔, 大野 典也, 亀田 治男
肝臓 29(5), 697-698 (1988)
Pubmed id:none 転移の治療実験
村上 孝司 済木育夫
実験医学 16(16), 162-167 (1998)
Pubmed id:none ヒト肝癌細胞JHH7からの geranylgeranyl pyrophosphate (GGPP) synthase cDNAの単離と発現に関する検討 新規な遺伝子機能アッセイ法の開発
三沢典彦
脂質生化学研究 41, 293-296 (1999)
Pubmed id:none 株化肝細胞癌の遺伝子発現 Gene expression in human hepatocellular carcinoma cell lines
村上 孝司 山田 雄次 済木 育夫 松浦 知和 蓮村 哲 永森 静志
組織培養研究 18(3), 221-228 (1999)
Pubmed id:none ヒト肝細胞癌由来株JHH-7の分子生物学的特徴とRAR-α特異的結合性を有する化合物TAC-101に対する反応性
大家 真治 村上 孝司 北里 健二
細胞 33(11), 32-35 (2001)
Pubmed id:none 転移の治療実験
村上 孝司 済木育夫
実験医学16(16),162-167 (1988)
Pubmed id:none ヒト肝細胞癌由来株JHH-7の分子生物学的特徴とRAR-α特異的結合性を有する化合物TAC-101に対する反応性
大家 真治 村上 孝司 北里 健二
細胞 33(11),32-35 (2001)

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LOT Information

Cell No.   JCRB1031 Cell Name   
LOT No.   10312012 Lot Specification   distribution
Medium   Williams' E medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   1.5 - 2.1 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 29 hrs. Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability (%)   98.4 Antibiotics Used   free
Passage Number   Unknown (10 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Williams' E CO2 Conc.   5%
RFLP    Additional information   
Cell No.   JCRB1031 Cell Name   
LOT No.   06252008 Lot Specification   distribution
Medium   William's E medium with 10% fetal bovine serum (GIBCO Cat.# 10099, lot 484375) Temperature   37 C
Cell Density at Seeding   approx. 2 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS. Subculture twice a week.
Doubling Time   30 hrs Cell Number in Vial (cells/1ml)   8.8 x 10^6
Viability (%)   96 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - William's E CO2 Conc.   5%
RFLP    Additional information   
Cell No.   JCRB1031 Cell Name   JHH-7
LOT No.   12212002 Lot Specification   distribution
Medium   Williams'E medium with 10% fetal bovine serum (Intergen RB52305). Temperature   37 C
Cell Density at Seeding   4.4 x 10^4 cells/sq.cm. Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Medium change once a week. Subculture once a week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.1 x 10^6
Viability (%)   99.0 Antibiotics Used   free
Passage Number   P4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium containing 5% DMSO. CO2 Conc.   NT
RFLP   unique Additional information   
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Cell No.   JCRB1031 Cell Name   JHH-7
LOT No.   01182016 Lot Specification   distribution
Medium   Williams' medium E with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091). Temperature   37 C
Cell Density at Seeding   approx. 2 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 26 hrs. Cell Number in Vial (cells/1ml)   4.8 x 10^6
Viability (%)   95.2 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Williams' medium E CO2 Conc.   5%
RFLP    Additional information   
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